Autophagy in mammalian systems
Author(s)
Bibliographic Information
Autophagy in mammalian systems
（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 452）
Elsevier, c2009
- pt. B
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Note
Includes bibliographical references and indexes
Description and Table of Contents
Description
This is the companion volume to Daniel Klionsky's Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, "Autophagy: From phenomenology to molecular understanding in less than a decade." He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy.
Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house cleaning" in the cell is called autophagy - literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth.
Table of Contents
Chapter 1
LC3-based assay for monitoring autophagy
Shunsuke Kimura, Naonobu Fujita, Takeshi Noda and Tamotsu Yoshimori
Chapter 2
Analysis of autophagy using GFP-LC3 transgenic mice
Noboru Mizushima
Chapter 3
Photoactivatable GFP
Jennifer Lippincott-Schwartz and Dale Hailey
Chapter 4
Assaying for autophagic protein degradation
Fred Meijer
Chapter 5
Sequestration assays for mammalian autophagy
Per O. Seglen
Chapter 6
Incorporation of monodansylcadaverine (MDC) as an assay to assess autophagy induction and monitoring fusion with a degradative compartment
Maria Isabel Colombo
Chapter 7
The GST-BHMT assay and related assays for autophagy
Carol A. Mercer and Patrick B. Dennis
Chapter 8
Redox and autophagy-ROS as an indicator for autophagic activity
Ruth Scherz-Shouval and Zvulun Elazar
Chapter 9
FACS analysis of autophagy
Elena Shvets, Ephraim Fass and Zvulun Elazar
Chapter 10
Electron microscopy in mammals
Paivi Yla-Anttila and Eeva-Liisa Eskelinen
Chapter 11
Monitoring mTOR activity
Ken Inoki
Chapter 12
Using p62 as a marker for autophagy.
Terje Johansen
Chapter 13
Cytosolic LC3 ratio as a quantitative index of macroautophagy
Motoni Kadowaki and Md. Razaul Karim
Chapter 14
Pexophagy in mammalian cells
Junji Ezaki,Takashi Ueno, Eiki Kominami and Masaaki Komatsu
Chapter 15
Mitophagy in mammalian cells.
Ji Zhang and Paul A. Ney
Chapter 16
Quantification of WIPI-1/Atg18 puncta formation for assessing mammalian autophagy
Tassula Proikas-Cezanne and Simon G. Pfisterer
Chapter 17
Correlative light and electron microscopy (CLEM) using GFP-LC3 and other autophagosome markers
Sharon Tooze and Minoo Razi
Chapter 18
Nanotechnology assays for autophagy
Oleksandr Seleverstov
Chapter 19
Photoconvertible KAEDA-LC3 for monitoring autophagic flux
Christoph Goemans and Aviva Tolkovsky
Chapter 20
Methods to monitor Chaperone-mediated autophagy
Ana Maria Cuervo and Susmita Kaushik
Chapter 21
Autophagy in response to Salmonella enterica serovar Typhimurium infection
Cheryl Birmingham and John Brumell
Chapter 22
Monitoring autophagy during Mycobacterium tuberculosis infection
Vojo Deretic
Chapter 23
Shigella, Listeria and Streptococcus induction of autophagy
Michinaga Ogawa,Yuko Yoshikawa, Ichiro Nakagawa,
Chihiro Sasakawa, and Trinad Chakraborty
Chapter 24
Measuring the impact of infection on autophagy in macrophages
Jean-Francois Dubuisson, Brenda Byrne and Michele Swanson
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