Chemistry and biochemistry of low molecular weight and protein thiols

著者

• Cadenas, Enrique
• Packer, Lester

書誌事項

Chemistry and biochemistry of low molecular weight and protein thiols

edited by Enrique Cadenas and Lester Packer

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 473 . Thiol redox transitions in cell signaling ; pt. A）

Academic Press, 2010

注記

Includes bibliographical references and indexes

内容説明・目次

内容説明

Thiol Redox Transitions in Cell Signaling, Part A, along with its companion (volume 475), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This first installment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylation and dethiolation, and peroxide removal by peroxiredoxins/thioredoxins and glutathione peroxidases. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

目次

His-tag switch method for the analysis of S-nitrosylated proteins Identification of Protein Thiols in Mitochondrial Oxidative Phosphorylation Complexes Mitochondrial thioredoxin reductase: purification, inhibitor studies, and role in cell signaling assessing cell surface thiol status Induction of Thioredoxin for Mediating Preconditioning-induced Cellular Responses A trans sarcoplasmic reticulum membrane redox sensor in the striated muscle: exploring redox sensitivity of the ryanodine receptor calcium release channel Rapid approach for the detection, quantification and discovery of novel sulphenic acid or S-nitrosothiol modified proteins using a biotin-switch method Direct identification by mass spectrometry of in vivo S-nitrosylated peptides Changing paradigms in theology: From antioxidant defence to redox regulation Determination of GSNO formation in biological samples by HPLC electrochemical detection Alteration of thioredoxin reductase 1 levels in elucidating cancer etiology thiol-labeling technology in proteomics Analytical methods for the determination of sulfur metabolite concentrations in cell extracts Chemical tagging and mass spectrometry-based identification of protein thiols modified by lipid peroxidation-derived a,b-unsaturated aldehydes Measuring protein thiol redox changes in mitochondria Engineering of redox domains for monitoring electron transfers spectroscopically Approaches to detection of cysteine sulfenic acids in proteins using dimedone-based chemical probes Evaluation of conditions affecting degree of sulfenic acid labeling in redox-sensitive proteins Colorimetric and spectrophotometric assays of sulfiredoxin Quantifying disulfides in specific proteins A simple method of detecting oxidatively-modified proteins Role of Glutathione Conjugates in Cell Signaling Regulation of Protein Function by Sulfinic Acid Formation Mass spectrometry approaches for the study of the oxidation state of protein cysteine residues assays of protein tyrosine phosphatase oxidation oxidation state of cellular 2-Cys peroxiredoxins (reduced thiol, disulfide and hyperoxidised) by non-reducing SDS-PAGE and immunoblotting

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詳細情報

• NII書誌ID(NCID): BB02362025
• ISBN:
  • 9780123813459
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: San Diego, Calif. ; Tokyo
• ページ数/冊数: xlviii, 359 p., [2] p. of plates
• 大きさ: 24 cm
• 親書誌ID:
  • BA00197543