Super-resolution, particle tracking, multiparameter, and force based methods

著者

• Walter, Nils G.

書誌事項

Super-resolution, particle tracking, multiparameter, and force based methods

edited by Nils G. Walter

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 475 . Single molecule tools ; pt. B）

Academic Press, 2010

注記

Includes bibliographical references and indexes

内容説明・目次

内容説明

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.

目次

1. Watching single DNA replication loops under flow extension Antoine Van Oijen 2. Star polymer surface passivation for single molecule detection Jurgen Groll , Martin Moeller 3. Ultrahigh resolution detection of single active motor proteins in live cells Paul Selvin, Erdal Toprak & Comert Kural 4. Molecules and Methods for Superresolution Imaging W.E. Moerner, Michael Thompson, Sam Lord, Julie Biteen, and Nick Conley +... 5. Aqueous nanodroplets for studying single molecules Lori Goldner 6. High-speed atomic force microscopy techniques for visualizing dynamic behavior of biological macromolecules Toshio Ando 7. Single-Biomolecule Spectroscopy Using Microfluidic Platforms Richard Zare, Dr. Olga Dudko, Dr. Jerome Mathe 8. DNA Looping Kinetics Analyzed by Tethered Particle Microscopy Laura Finzi 9. Single molecule observation of proteins in vivo Shimon Weiss, Gopal Iyer 10. DNA curtains as a high-throughput approach to single molecule imaging Eric Greene 11. Single-molecule enzymology of protein synthesis Ruben Gonzalez 12. Single molecule fluorescence studies of intrinsically disordered proteins Ashok Deniz 13. Nanovesicle trapping for studying transient protein-protein interactions by single molecule FRET Peng Chen 14. Tracking single motor proteins in the cytoplasm of mammalian cells Edgar Meyhoefer 15. Conformational States of F1-ATPase by Single-Molecule Rotation Hiroyuki Noji 16. Single Molecule Sequencing by Fluorescence Imaging Patrice Milos 17. Real-Time DNA Sequencing from Single Polymerase Molecules Jonas Korlach 18. Micropatterning and single molecule imaging for quantitative analysis of protein-protein interactions in living cells Gerhard Schutz 19. Probing virus-receptor interactions by atomic force spectroscopy Peter Hinterdorfer 20. Single-Molecule Fluorescence Spectroscopy of Cytochrome P450 in Nanodiscs Elizabeth Rhoades, William M. Atkins 21. Analysis of complex single molecule FRET time traces Nils Walter, Mario Blanco 22. Application of super-resolution imaging to single particle tracking in nanotechnology Nils Walter, Chamaree de Silva & Anthony Manzo 23. Scanning FCS for the characterization of protein dynamics in live cells Petra Schwille 24. Single mRNA molecule tracking in live cells Rob Singer 25. Single-molecule high-resolution colocalization (SHREC) or Single-molecule optical-trap analyses of protein structure James Spudich 26. Nanopore force Spectroscopy tools for analyzing single bio-complexes Amit Meller 27. Use of plasmon coupling to reveal DNA dynamics at the single molecule level Jan Liphardt 28. "Fluorescence-force spectroscopy" or "Suppression of fluorophore blinking and bleaching" or "Spontaneous intersubunit rotation of the ribosome" Taekjip Ha 29. Multiplexed single mRNA imaging in fixed cells Sanjay Tyagi, Arjun Raj 30. Size-Minimized Quantum Dots for Single-Molecule and Intracellular Imaging Shuming Nie 31. The ABEL trap Adam Cohen, Alex Fields 32. An optical torque wrench Steve Block, Arthur La Porta 33. Determining the Stoichiometry of Protein Hetero-complexes in Living Cells with Fluorescence Fluctuation Spectroscopy Joachim Mueller 34. Fluorescent Visualization of Single Protein-DNA Complexes Steve Kowalzcykowski 35. Direct Measurement of Tertiary Contact Cooperativity in RNA Folding by single molecule FRET Daniel Herschlag 36. Nanometer-localized multiple single-molecule (NALMS) or Single-molecule nonequilibrium periodic Mg2+-concentration jump experiments Norbert Scherer 37. Multiparameter single molecule fluorescence detection with applications to FRET Claus Seidel 38. single-particle tracking-photoactivated localization microscopy (sptPALM) within live cells Jennifer Lippincott-Schwartz, Suliana Manley 39. Site-specific incoporation of fluorescent probes into RNA polymerase Richard Ebright 40. Quantitative single-molecule imaging by confocal laser scanning microscopy Rudolph Rigler 41. Studies of DNA-replication at the single molecule level using magnetic tweezers David Bensimon, Vincent Croquette 42. RNA labeled for single molecule FRET analysis from ligation with T4 RNA ligases John Abelson 43. Combining optical tweezers, single-molecule fluorescence microscopy and microfluidics for studying reversible protein-DNA interactions Gijs Wuite, G. Farge, P. Gross & E.J.G. Peterman 44. How dwell time distributions and other such observables in single molecule analysis can be used to extract information from molecular systems Carlos Bustamante, Jeff Moffitt & Yann Robert Chemla 45. Atomic force microscopy studies of human rhinovirus: topology and molecular forces Peter Hinterdorfer

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詳細情報

• NII書誌ID(NCID): BB02943935
• ISBN:
  • 9780123814821
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: San Diego, Calif. ; Tokyo
• ページ数/冊数: l, 663 p., [30] p. of plates
• 大きさ: 24 cm
• 分類:
  • DC22 : 572.7
• 件名:
  • LCSH : Enzymology
  • LCSH : Molecular biology
  • LCSH : Ultrastructure (Biology)
• 親書誌ID:
  • BA00197543