Mouse molecular genetics

Author(s)

• Wassarman, Paul M.
• Soriano, Philippe M.

Bibliographic Information

Mouse molecular genetics

edited by Paul M. Wassarman and Philippe M. Soriano

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 477 . Guide to techniques in mouse development ; pt. B）

Academic Press, 2010

2nd ed

• : hardback
• : pbk

Note

Includes bibliographical references and indexes

Description and Table of Contents

Volume

: hardback ISBN 9780123848802

Description

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development

Table of Contents

Section I. Transgenesis 1. Lentivirus transgenesis Alexander Pfeifer and Katrin Zimmermann 2. Germline modification using mouse spermatogonial stem cells Mito Kanatsu-Shinohara and Takashi Shinohara 3. Embryonic in vivo electroporation in the mouse Tetsuichiro Saito Section II. Transposons 4. Current applications of transposons in mouse genetics Adam J. Dupuy 5. Functional genomics in the mouse using the Sleeping Beauty transposon system Kyoji Horie, Chikara Kokubu, and Junji Takeda 6. The use of DNA transposons for cancer gene discovery in mice George Vassiliou, Roland Rad, and Allan Bradley Section III. Recombinases 7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2 Konstantinos Anastassiadis, Stefan Glaser, Andrea Kranz, Kaj Berhardt, and A. Francis Stewart 8. A recombineering pipeline to make conditional targeting constructs Jun Fu, Madeleine Teucher, Konstantinos Anastassiadis, William Skarnes, and A. Francis Stewart 9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria M. David Stewart and Richard R. Behringer 10.Genetic fate mapping using site-specific recombinases Emilie Legue and Alexandra L. Joyner 11. Mapping cell fate and function using recombinase-based intersectional strategies Susan M. Dymecki, Russell S. Ray, and Jun C. Kim Section IV. Mutagenesis 12. Genome-wide forward genetic screens in mouse ES cells Meng Amy Li, Stephen J. Pettitt, Kosuke Yusa, and Allan Bradley 13. Gene trap mutagenesis in the mouse Roland H. Friedel and Philippe Soriano 14. A wider context for gene trap mutagenesis Joshua M. Brickman, Anestis Tsakiridis, Christine To, and William L. Stanford 15. Mouse mutagenesis with the chemical supermutagen ENU Frank J. Probst and Monica J. Justice 16. Phenotype-driven mouse ENU mutagenesis screens Tamara Caspary 17. Using ENU mutagenesis for phenotype-driven analysis of the mouse. Rolf W. Stottmann and David R. Beier Section V. Gene Knockdowns 18. Exploration of self-renewal and pluripotency in ES cells using RNAi Christoph Schaniel, Dung-Fang Lee, and Ihor R. Lemischka 19. Transgenic RNAi applications in the mouse Jost Seibler and Frieder Schwenk 20. Gene knockdown in the mouse through RNAi Aljoscha Kleinhammer, Wolfgang Wurst, and Ralf Kuhn 21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice Christopher S. Raymond, Lei Zhu, and Myung K. Shin 22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription Kai Schoenig, Hermann Bujard, and Manfred Gossen Section VI. Gene Expression Profiling 23. Gene expression profiling of mouse oocytes and preimplantation embryos Francesca E. Duncan and Richard M. Schultz 24. Interrogating the transcriptome of oocytes and preimplantation embryos Anne E. Peaston, Joel Graber, Barbara B. Knowles, and Wilhelmine N. de Vries 25. Gene expression profiling of mouse embryos with microarrays Alexei A. Sharov, Yulan Piao, and Minoru S. H. Ko

Volume

: pbk ISBN 9780123848826

Description

Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology. The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text: Lentivirus transgenesis o Germline modification using mouse stem cells Electroporation Applications of transposons in mouse genetics Functional genomics using transposon systems The use of DNA transposons in detecting cancer genes in mice Recombination, conditional mutagenesis and induction of tamoxifen Genetic fate mapping using recombinases Genetic screens mouse ES cells Gene trap mutagenesis Mouse mutagenesis Self- renewal and pluripotency Transgenic RNAi applications Gene knockdowns Tetracycline-controlled transcription Gene expression profiling of mouse embryos The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice.

Table of Contents

Section I. Transgenesis1. Lentivirus transgenesis2. Germline modification using mouse spermatogonial stem cells3. Embryonic in vivo electroporation in the mouse Section II. Transposons4. Current applications of transposons in mouse genetics5. Functional genomics in the mouse using the Sleeping Beauty transposon system6. The use of DNA transposons for cancer gene discovery in mice Section III. Recombinases7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT28. A recombineering pipeline to make conditional targeting constructs 9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria10.Genetic fate mapping using site-specific recombinases11. Mapping cell fate and function using recombinase-based intersectional strategies Section IV. Mutagenesis12. Genome-wide forward genetic screens in mouse ES cells13. Gene trap mutagenesis in the mouse14. A wider context for gene trap mutagenesis 15. Mouse mutagenesis with the chemical supermutagen ENU16. Phenotype-driven mouse ENU mutagenesis screens17. Using ENU mutagenesis for phenotype-driven analysis of the mouse Section V. Gene Knockdowns18. Exploration of self-renewal and pluripotency in ES cells using RNAi19. Transgenic RNAi applications in the mouse 20. Gene knockdown in the mouse through RNAi21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription Section VI. Gene Expression Profiling23. Gene expression profiling of mouse oocytes and preimplantation embryos24. Interrogating the transcriptome of oocytes and preimplantation embryos25. Gene expression profiling of mouse embryos with microarrays

by "Nielsen BookData"

Details

• NCID: BB03117923
• ISBN:
  • 9780123848802
  • 9780123848826
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: San Diego, Calif. ; Tokyo
• Pages/Volumes: xlviii, 580 p., [8] p. of plates
• Size: 24 cm
• Classification:
  • DC22 : 571.61935
• Subject Headings:
  • LCSH : Mice -- Cytology
  • LCSH : Mice -- Embryology
  • LCSH : Mice -- Development
• Parent Bibliography ID:
  • BA00197543