Sample preparation and data collection

著者

• Jensen, Grant J.

書誌事項

Sample preparation and data collection

edited by Grant J. Jensen

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 481 . Cryo-EM ; pt. A）

Academic Press, 2010

• : hardcover

注記

Includes bibliographical references and indexes

内容説明・目次

内容説明

Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

目次

Historical perspective 3D reconstruction from electron micrographs: a personal account of its development David DeRosier Preparation of 2-D crystals Henning Stahlberg Helical Crystallization of Soluble and Membrane Binding Proteins Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie Plunge Freezing for Electron Cryomicroscopy Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen A Practical Guide to the Use of Monolayer Purification and Affinity Grids Thomas Walz, Deborah F. Kelly, Danijela Dukovski GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM Holger Stark Cryo-negative staining of macromolecular assemblies Sacha De Carlo, Holger Stark Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM Fred J. Sigworth, Liguo Wang Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing Mark S. Ladinsky Site-Specific Biomolecule Labeling with Gold Clusters Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld How to Operate a Cryo-Electron Microscope Huilin Li, Jingchuan Sun Collecting electron crystallographic data of two-dimensional protein crystals Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng Automated Data Collection for Electron Microscopic Tomography David Agard, Shawn Q. Zheng, J. W. Sedat Correlated Light and Electron Cryomicroscopy Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jurgen M. Plitzkod, Cindi L. Schwartz Phase Plates for Transmission Electron Microscopy Radostin Danev, Kuniaki Nagayama Radiation damage in electron cryomicroscopy John L. Rubinstein, Lindsay A. Baker

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詳細情報

• NII書誌ID(NCID): BB03572307
• ISBN:
  • 9780123749062
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: San Diego, Calif. ; Tokyo
• ページ数/冊数: xlv, 410 p., [10] p. of plates
• 大きさ: 24 cm
• 分類:
  • DC22 : 502.825
• 件名:
  • LCSH : Electron microscopy -- Technique
• 親書誌ID:
  • BA00197543