Antigen retrieval immunohistochemistry based research and diagnostics

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書誌事項

Antigen retrieval immunohistochemistry based research and diagnostics

edited by Shan-Rong Shi, Clive R. Taylor

（Wiley series in biomedical engineering and multi-disciplinary integrated systems, [1]）

John Wiley & Sons, c2010

  • :hardback

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注記

Summary: "This book is based on the development and application of AR by the editors, one of whom is the inventor of AR, together with members of a world-leading research center of AR"-- Provided by publisher

内容説明・目次

内容説明

The most complete, up-to-date reference on antigen retrieval and immunohistochemistry An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects. Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work: Summarizes the key problems in the four fields of antigen retrieval Discusses the advances of AR techniques and their applications Provides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell/tissue sample preparation, and standardization and development of various techniques to meet the future needs of?clinical and research molecular analysis Encourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC Includes an appendix of related laboratory protocols Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products. Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor

目次

Preface ix Contributors xv Part I Recent Advances in Antigen Retrieval Techniques and Its Application 1 1 Standardization of Antigen Retrieval Techniques Based on the Test Battery Approach 3 Shan-Rong Shi and Clive R. Taylor 2 Extended Application of Antigen Retrieval Technique in Immunohistochemistry and in Situ Hybridization 25 Shan-Rong Shi and Clive R. Taylor 3 Extraction of Dna/rna From Formalin-fixed, Paraffin-embedded Tissue Based on the Antigen Retrieval Principle 47 Shan-Rong Shi and Clive R. Taylor Part II Standardization of Immunohistochemistry 73 4 Key Issues and Strategies of Standardization For Quantifiable Immunohistochemistry 75 Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor 5 Standardization of Immunohistochemistry Based on Antigen Retrieval Technique 87 Shan-Rong Shi and Clive R. Taylor 6 Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of Her2 Ihc and Ish Testing 101 Bharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim, and Keith Miller 7 Peptides as Immunohistochemistry Controls 123 Steven A. Bogen and Seshi R. Sompuram 8 Standard Reference Material: Protein- Embedding Technique and Design of Bar Code 141 Shan-Rong Shi, Jiang Gu, and Clive R. Taylor 9 the Pros and Cons of Automation for Immunohistochemistry From the Prospective Of the Pathology Laboratory 151 David G. Hicks and Loralee McMahon 10 Image Analysis in Immunohistochemistry 165 Alton D. Floyd Part III Tissue/cell Sample Preparation 187 11 Tissue Cell Sample Preparation: Lessons From the Antigen Retrieval Technique 189 Shan-Rong Shi and Clive R. Taylor 12 Mechanisms of Action and Proper Use of Common Fixatives 195 Richard W. Dapson 13 Cell Sample Preparation for Clinical Cytopathology: Current Status and Future Development 219 Yan Shi and Patricia G. Wasserman 14 Design of a Tissue Surrogate to Examine Accuracy of Proteomic Analysis 235 Carol B. Fowler, Jeffrey T. Mason, and Timothy J. O'leary Part IV Molecular Mechanism of Antigen Retrieval Technique 251 15 Study of Formalin Fixation and Heat-induced Antigen Retrieval 253 Jeffrey T. Mason, Carol B. Fowler, and Timothy J. O'leary 16 a Linear Epitopes Model of Antigen Retrieval 287 Steven A. Bogen and Seshi R. Sompuram 17 Ph Or Ionic Strength of Antigen Retrieval Solution: a Potential Role for Refolding During Heat Treatment 303 Shuji Yamashita 18 Commentary: Future Directions 323 Timothy J. O'Leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason Part V Proteomic Analysis of Protein Extracted From Tissue/cells 333 19 Techniques of Protein Extraction From Ffpe Tissue/cells for Mass Spectrometry 335 Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason 20 Application of Shotgun Proteomics to Formalin-fixed and Paraffin-embedded Tissues 347 Brian M. Balgley 21 Visualizing Protein Maps in Tissue 369 Masahiro Mukai and Mitsutoshi Setou 22 Symbiosis of Immunohistochemistry and Proteomics: Marching to a New Era 391 Shan-Rong Shi, Brian M. Balgley, and Clive R. Taylor Appendix Related Laboratory Protocols 399 Index 413

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