Chloroplast research in arabidopsis : methods and protocols

著者

• Jarvis, R. Paul

書誌事項

Chloroplast research in arabidopsis : methods and protocols

edited by R. Paul Jarvis

（Springer protocols）（Methods in molecular biology / John M. Walker, series editor, 774-775）

Humana Press, c2011

• v. 1 : [cased]
• v. 2 : [cased]

注記

Includes bibliographical references and index

内容説明・目次

巻冊次

v. 1 : [cased] ISBN 9781617792335

内容説明

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume I explores topics such as genetics, cytology, in vivo analysis, gene expression, and protein accumulation, as well as protein transport, localization, and topology. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

目次

Part I: Genetics, Cytology, and In Vivo Analysis 1. Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis Enrique Lopez-Juez and Alison Hills 2. Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy Kevin Pyke 3. Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins Stanislav Vitha and Katherine W. Osteryoung 4. Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts Dong Wook Lee and Inhwan Hwang 5. Visualisation of Stromules on Arabidopsis Plastids John C. Gray, James A. Sullivan, and Christine A. Newell 6. Analysis of Chloroplast Movement and Relocation in Arabidopsis Masamitsu Wada and Sam-Geun Kong 7. Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots John Stanga, Allison Strohm, and Patrick H. Masson 8. Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy Stefan Hyman and R. Paul Jarvis 9. Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga Part II: Gene Expression and Protein Accumulation 10. Isolation, Quantification, and Analysis of Chloroplast DNA Beth A. Rowan and Arnold J. Bendich 11. Measurement of Transcription Rates in Arabidopsis Chloroplasts Yan O. Zubo, Thomas Boerner, and Karsten Liere 12. Studying the Structure and Processing of Chloroplast Transcripts Alice Barkan 13. In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts Toshiharu Shikanai and Kenji Okuda 14. Studying Translation in Arabidopsis Chloroplasts Paolo Pesaresi 15. Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts Lars L.E. Sjoegren and Adrian K. Clarke Part III: Protein Transport, Localization, and Topology 16. In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins Sandra Tanz and Ian Small 17. Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays Henrik Aronsson and R. Paul Jarvis 18. Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions Hitoshi Inoue, Fei Wang, Takehito Inaba, and Danny J. Schnell 19. Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays Thomas Bals and Danja Schunemann 20. Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction Chiung-Chih Chu and Hsou-min Li 21. Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases John Froehlich

巻冊次

v. 2 : [cased] ISBN 9781617792366

内容説明

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

目次

Part I: Multiprotein Complexes and Protein-Protein Interactions 1. One- and Two-Dimensional Blue Native (BN) PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes Shingo Kikuchi, Jocelyn Bedard, and Masato Nakai 2. Analysis of Thylakoid Protein Complexes by Two Dimensional Electrophoretic Systems Sari Sirpioe, Marjaana Suorsa, and Eva-Mari Aro 3. Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification Charles Andres, Birgit Agne, and Felix Kessler 4. Studying Interactions between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation (BiFC) and Foerster Resonance Energy Transfer (FRET) Jodi Maple and Simon G. Moller 5. Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods Joanna Tripp and Enrico Schleiff Part II: Omics and Large-Scale Analyses 6. Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis Diogo Ribeiro Demartini, Celia Regina Carlini, and Jay J. Thelen 7. Use of Transcriptomics to Analyse Chloroplast Processes in Arabidopsis Tatjana Kleine and Dario Leister 8. Use of Non-Aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis Peter Geigenberger, Axel Tiessen, and Joerg Meurer 9. Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis Yan Lu, Linda J. Savage, and Robert L. Last Part III: Proteomics and Suborganellar Fractionation 10. Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies Daniel Salvi, Lucas Moyet, Daphne Seigneurin-Berny, Myriam Ferro, Jacques Joyard, and Norbert Rolland 11. Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis Michael Hall, Yogesh Mishra, and Wolfgang P. Schroeder 12. Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies Celine Besagni, Lucia Eugeni Piller, and Claire Brehelin 13. Preparation and Proteomic Analysis of Chloroplast Ribosomes Kenichi Yamaguchi 14. The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting Giulia Friso, Paul Dominic B. Olinares, and Klaas J. van Wijk 15. Use of Phosphoproteomics to Study Post-Translational Protein Modifications in Arabidopsis Chloroplasts Anne Endler and Sacha Baginsky Part IV: Photosynthesis and Biochemical Analysis 16. Use of a Pulse-Amplitude Modulated (PAM) Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis in Arabidopsis Plants Matthew D. Brooks and Krishna K. Niyogi 17. Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves Giles Johnson and Erik Murchie 18. Measurement of the pH and Electric Field Developed Across Arabidopsis Thylakoids in the Light Steven M. Theg and Curtis Tom 19. Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis Aleel K. Grennan and Donald R. Ort 20. Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis Olaf Czarnecki, Enrico Peter, and Bernhard Grimm 21. Analysis of Starch Metabolism in Chloroplasts Carmen Hostettler, Katharina Koelling, Diana Santelia, Sebastian Streb, Oliver Koetting, and Samuel C. Zeeman 22. The Analysis of Lipid Content and Quality in Arabidopsis Plastids Anna Maria Zbierzak, Peter Doermann, and Georg Hoelzl

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詳細情報

• NII書誌ID(NCID): BB06936534
• ISBN:
  • 9781617792335
  • 9781617792366
• LCCN: 2011932678
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: v.
• 大きさ: 26 cm
• 分類:
  • DC22 : 571.6592364
• 件名:
  • LCSH : Chloroplasts -- Research -- Methodology
  • LCSH : Arabidopsis -- Molecular aspects
• 親書誌ID:
  • BA88873632
  • BA10794673