Spermatogenesis : methods and protocols
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書誌事項
Spermatogenesis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 927）
Humana Press, [2012], c2013
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.
目次
Section I. Semen Analysis Methods
1. Methods For Sperm Concentration Determination
Lars Bjoerndahl
2. Methods of Sperm Vitality Assessment
Sergey I. Moskovtsev and Clifford L. Librach
3. The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity
Sivakumar Ramu and Rajasingam S. Jeyendran
4. Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization
Susan A. Rothmann, Anna-Marie Bort, John Quigley, and Robin Pillow
5. Sperm Morphology Assessment Using Strict (Tygerberg) Criteria
Roelof Menkveld
6. Methods For Direct And Indirect Antisperm Antibody Testing
Hiroaki Shibahara and Junko Koriyama
7. Manual Methods For Sperm Motility Assessment
David Mortimer and Sharon T. Mortimer
8. Computer-Aided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation
David Mortimer and Sharon T. Mortimer
Section II. Tests of Sperm Function
9. The Hemizona Assay For Assessment Of Sperm Function
Sergio Oehninger, Mahmood Morshedi, and Daniel Franken
10. The Sperm Penetration Assay for the Assessment of Fertilization Capacity
Kathleen Hwang and Dolores J. Lamb
11. Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay
Christopher J. De Jonge and Christopher L. R. Barratt
Section III. Sperm DNA Damage Testing
12. Sperm DNA Fragmentation Analysis Using the TUNEL Assay
Rakesh Sharma, Jayson Masaki, and Ashok Agarwal
13. Sperm DNA Damage Measured by Comet Assay
Luke Simon and Douglas T. Carrell
14. Sperm Chromatin Structure Assay (SCSA (R))
Donald P. Evenson
Section IV. Advanced Clinical Testing
15. Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH)
Benjamin R. Emery
16. Flow Cytometric Methods For Sperm Assessment
Vanesa Robles and Felipe Martinez-Pastor
17. Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions
Peter H. Vogt and Ulrike Bender
Section V. Sperm Preparation and Selection Techniques
18. Sperm Cryopreservation Methods
Tiffany Justice and Greg Christensen
19. Density Gradient Separation Of Sperm For Artificial Insemination
David Mortimer and Sharon T. Mortimer
20. Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis
Charles H. Muller and Erin R. Pagel
21. Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor
Shilo L. Archer and William E. Roudebush
22. Intracytoplasmic Morphology Selected Sperm Injection
Luke Simon, Aaron Wilcox, and Douglas T. Carrell
23. Sperm Selection For ICSI Using Annexin V
Sonja Grunewald and Uwe Paasch
24. Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay
Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh
25. Sperm Selection Based On Electrostatic Charge
Luke Simon, Shao-qin Ge, and Douglas T. Carrell
26. Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting
Duane L. Garner, K. Michael Evans, and George E. Seidel, Jr.
Section VI. Staging and Histology Techniques
27. Assessment of Spermatogenesis Through Staging of Seminiferous Tubules
Marvin L. Meistrich and Rex A. Hess
28. Immunohistochemical Approaches for the Study of Spermatogenesis
Cathryn A. Hogarth and Michael D. Griswold
29. Ultrastructural Analysis of Testicular Tissue and Semen by Transmission and Scanning Electron Microscopy
Hector E. Chemes
Section VII. Oxidative Stress Testing
30. Assessment Of Oxidative Stress In Sperm And Semen
Anthony H. Kashou, Rakesh Sharma, and Ashok Agarwal
31. Improved Chemiluminescence Assay for Measuring Antioxidant Capacity of Seminal Plasma
Charles H. Muller, Tiffany K. Y. Tang, and Michalina A. Montano
Section VIII. Analytical Tools and Methods
32. Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies
Jeanine Griffin
33. Isolating mRNA and Small Noncoding RNAs From Human Sperm
Robert J. Goodric, Ester Anton, and Stephen A. Krawetz
34. A Review Of Genome-Wide Approaches To Study The Genetic Basis For Spermatogenic Defects
Kenneth I. Aston and Donald F. Conrad
35. Methods For The Analysis Of The Sperm Proteome
Sara de Mateo, Josep Maria Estanyol, and Rafael Oliva
Section IX. Methodologies to Evaluate Sperm Epigenetics
36. Methodology of Aniline Blue Staining Of Chromatin and the Assessment of the Associated Nuclear and Cytoplasmic Attributes in Human Sperm
Leyla Sati and Gabor Huszar
37. Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents
W. Steven Ward
38. Extraction and Analysis of Human Sperm Protamine 1 / Protamine 2 Ratio Using Acid Gel Electrophoresis
Lihua Liu, Kenneth I. Aston, and Douglas T. Carrell
39. Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation
Saher Sue Hammoud, Bradley R. Cairns, and Douglas T. Carrell
40. Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm
David Miller and Agnieszka Paradowska
Section X. In Vitro and Ex Vivo Spermatogenesis Models
41. In Vitro Spermatogenesis Using An Organ Culture Technique
Tetsuhiro Yokonishi, Takuya Sato, Kumiko Katagiri, and Takehiko Ogawa
42. Testicular Tissue Grafting and Male Germ Cell Transplantation
Jose R. Rodriguez-Sosa, Lin Tang, and Ina Dobrinski
Section XI. Transgenic Techniques
43. Transgenic Modification of Spermatogonial Stem Cells Using Lentiviral Vectors
Christina Tenenhaus Dann
44. Methods for Sperm-Mediated Gene Transfer
Marialuisa Lavitrano, Roberto Giovannoni, and Maria Grazia Cerrito.
45. Phenotypic Assessment of Male Fertility Status in Transgenic Animal Models
David M. de Kretser and Liza O'Donnell
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