Manipulating the mouse embryo : a laboratory manual

Author(s)

Bibliographic Information

Manipulating the mouse embryo : a laboratory manual

Richard Behringer ... [et al.]

Cold Spring Harbor Laboratory Press, c2014

4th ed

  • : cloth
  • : pbk

Available at  / 52 libraries

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Note

Includes bibliographical references and index

Other authors: Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Contents of Works

  • Genetics and embryology of the mouse: past, present, and future
  • Summary of mouse development
  • A mouse colony for the production of transgenic and chimeric animals
  • Recovery and in vitro culture of preimplantation embryos
  • Isolation, culture, and manipulation of postimplantation embryos
  • Surgical procedures
  • Production of transgenic mice by pronuclear microinjection
  • Embryo-derived stem cell lines
  • Germline-competent stem cells derived from adult mice
  • Vector designs for pluripotent stem cell-based transgenesis and genome alterations
  • Introduction of foreign DNA into embryonic stem cells
  • Production of chimeras
  • Genotyping
  • Parthenogenesis, pronuclear transfer, and mouse cloning
  • Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection
  • Cryopreservation, rederivation, and transport of mouse strains
  • Techniques for visualizing gene products, cells, tissues, and organ systems
  • Setting up a micromanipulation lab

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