Manipulating the mouse embryo : a laboratory manual
著者
書誌事項
Manipulating the mouse embryo : a laboratory manual
Cold Spring Harbor Laboratory Press, c2014
4th ed
- : cloth
- : pbk
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注記
Includes bibliographical references and index
Other authors: Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy
収録内容
- Genetics and embryology of the mouse: past, present, and future
- Summary of mouse development
- A mouse colony for the production of transgenic and chimeric animals
- Recovery and in vitro culture of preimplantation embryos
- Isolation, culture, and manipulation of postimplantation embryos
- Surgical procedures
- Production of transgenic mice by pronuclear microinjection
- Embryo-derived stem cell lines
- Germline-competent stem cells derived from adult mice
- Vector designs for pluripotent stem cell-based transgenesis and genome alterations
- Introduction of foreign DNA into embryonic stem cells
- Production of chimeras
- Genotyping
- Parthenogenesis, pronuclear transfer, and mouse cloning
- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection
- Cryopreservation, rederivation, and transport of mouse strains
- Techniques for visualizing gene products, cells, tissues, and organ systems
- Setting up a micromanipulation lab