Methods of adipose tissue biology

Author(s)

• Macdougald, Ormond A.

Bibliographic Information

Methods of adipose tissue biology

edited by Ormond A. Macdougald

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 537-538）

Elsevier/Academic Press, 2014

• Pt. A
• Pt. B

Note

Includes bibliographical references and indexes

Description and Table of Contents

Volume

Pt. A ISBN 9780124116191

Description

Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.

Table of Contents

1. Adipocyte-Specific Transgenic and Knockout Models 2. Imaging White Adipose Tissue With Confocal Microscopy 3. Isolation and Study of Adipocyte Precursors 4. Imaging of Adipose Tissue 5. Adipose Tissue Angiogenesis Assay 6. Quantifying Size and Number of Adipocytes in Adipose Tissue 7. Use of Osmium Tetroxide Staining with Micro-Computerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue in Vivo 8. Brown Adipose Tissue in Humans: Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography) 9. Analysing the Functions and Structure of the Human Lipodystrophy Protein Seipin 10. Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes 11. Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue 12. Measurement and manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion 13. Isolation and Quantitation of Adiponectin Higher Order Complexes 14. Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq 15. Analysis and Isolation of Adipocytes by Flow Cytometry 16. Flow Cytometry Analyses of Adipose Tissue Macrophages

Volume

Pt. B ISBN 9780128002803

Description

This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.

Table of Contents

1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice 2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover 3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells 4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells 5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation 6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry 7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes 8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity 9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes 10. Measurement of Lipolysis 11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics 12. Methods for Performing Lipidomics in White Adipose Tissue 13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time 14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes 15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis 16. Evaluation of Protein Phosphorylation during Adipogenesis

by "Nielsen BookData"

Details

• NCID: BB15128723
• ISBN:
  • 9780124116191
  • 9780128002803
• Country Code: ne
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Amsterdam ; Tokyo
• Pages/Volumes: 2 v.
• Size: 24 cm
• Classification:
  • DC23 : 611.01827
• Subject Headings:
  • LCSH : Adipose tissues
  • LCSH : Adipose tissues -- Histology
  • LCSH : Histology -- Technique
• Parent Bibliography ID:
  • BA00197543