Methods of adipose tissue biology
著者
書誌事項
Methods of adipose tissue biology
(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 537-538)
Elsevier/Academic Press, 2014
- Pt. A
- Pt. B
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注記
Includes bibliographical references and indexes
内容説明・目次
- 巻冊次
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Pt. A ISBN 9780124116191
内容説明
Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.
目次
1. Adipocyte-Specific Transgenic and Knockout Models
2. Imaging White Adipose Tissue With Confocal Microscopy
3. Isolation and Study of Adipocyte Precursors
4. Imaging of Adipose Tissue
5. Adipose Tissue Angiogenesis Assay
6. Quantifying Size and Number of Adipocytes in Adipose Tissue
7. Use of Osmium Tetroxide Staining with Micro-Computerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue in Vivo
8. Brown Adipose Tissue in Humans: Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography)
9. Analysing the Functions and Structure of the Human Lipodystrophy Protein Seipin
10. Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes
11. Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue
12. Measurement and manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion
13. Isolation and Quantitation of Adiponectin Higher Order Complexes
14. Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq
15. Analysis and Isolation of Adipocytes by Flow Cytometry
16. Flow Cytometry Analyses of Adipose Tissue Macrophages
- 巻冊次
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Pt. B ISBN 9780128002803
内容説明
This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.
目次
1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice
2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover
3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells
4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells
5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation
6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry
7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes
8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity
9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes
10. Measurement of Lipolysis
11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics
12. Methods for Performing Lipidomics in White Adipose Tissue
13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time
14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes
15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis
16. Evaluation of Protein Phosphorylation during Adipogenesis
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