Electron microscopy : methods and protocols
Author(s)
Bibliographic Information
Electron microscopy : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1117）（Springer protocols）
Humana Press, c2014
3rd ed. / edited by John Kuo
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Note
Previously published in 2007
Includes bibliographical references and index
Description and Table of Contents
Description
This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.
Table of Contents
1. Conventional Specimen Preparation Techniques For Transmission Electron Microscopy of Cultured Cells
John J. Bozzola
2. Microwave-assisted Processing and Embedding for Transmission Electron Microscopy
Paul Webster
3. Processing Plant Tissues for Ultrastructural Study John Kuo
4. Staining Sectioned Biological Specimens for Transmission Electron Microscopy:
Conventional and En Bloc Stains
E. Ann Ellis
5. Metal Shadowing for Electron Microscopy
Gregory M. Hendricks
6. Freeze Fracture and Freeze Etching
Douglas E. Chandler and William P. Sharp
7. Conventional Specimen Preparation Techniques For Scanning Electron Microscopy of Biological Specimens
John J. Bozzola
8. High-pressure Freezing: Current State and Future Prospects
Andres Kaech and Urs Ziegler
9. Cryo-fixation by Self-pressurized Rapid Freezing Markus Grabenbauer, Hong-Mei Han, and Jan Huebinger
10. Cryo-Electron Microscopy of Vitreous Sections
Petr Chlanda and Martin Sachse
11. Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine
J. Robin Harris
12. Electron Microscopy of the Microtubule Cytoskeleton Assembly Vitro
Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison
13. Cryosectioning Fixed and Cryoprotected Biological Material for Immmunocytochemistry
Paul Webster and Alexandre Webster
14. Analysis of Specificity in Immunoelectron Microscopy
Christian Hacker and John M. Lucocq
15. Cryo-Electron Microscopy of Membrane Proteins
Kenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, and Henning Stahlberg
16. Tracking DNA and RNA Sequences at High Resolution
Dusan Cmarko, Anna Ligasova, and Karel Koberna
17. Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy
Yuri L. Lyubchenko, Alexander A. Gall, and Luda S. Shlyakhtenko
18. Biological Applications of Phase-Contrast Electron Microscopy
Kuniaki Nagayama
19. Single Particle Cryo-Electron Microscopy And 3-D Reconstruction Of Viruses
Fei Guo and Wen Jiang
20. Electron Tomography for Organelles, Cells and Tissues
Wanzhong He and Yongning He
21. Correlative Light and Electron Microscopy: from Live Cell Dynamic to 3D Ultrastructure
Coralie Spiegelhalter, Jocelyn F. Laporte, and Yannick Schwab
22. Nanometer-resolution Fluorescence Electron Microscopy (Nano-EM) in Cultured Cells
Shigeki Watanabe, Martin Lehmann, Edward Hujber, Richard D. Fetter, Jackson Richards, Berit Soehl-Kielczynski, Annegret Felies, Christian Rosenmund, Jan Schmoranzer, and Erik M. Jorgensen
23. Correlative Fluorescence- and Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid
Diana B. Peckys, Madeline J. Dukes, and Niels de Jonge
24. FIB-SEM Tomography in Biology
Caroline Kizilyaprak
25. Correlative Light and Electron Microscopy using Immunolabeled Sections
Heinz Schwarz and Bruno M. Humbel
26. Correlative 3D Imaging: CLSM and FIB-SEM Tomography Using High-Pressure Frozen, Freeze-Substituted Biological Samples
Miriam S. Lucas, Maja Guenthert, Philippe Gasser, Falk Lucas and Roger Wepf
27. Three-dimensional Imaging of Adherent Cells using FIB-SEM and STEM
Clarissa Villinger, Martin Schauflinger, Heiko Gregorius, Christine Kranz, Katharina Hoehn, Soufi Nafeey, Paul Walther
28. X-Ray Microanalysis in the Scanning Electron MicroscopeGodfried M. Roomans and Anca Dragomir
29. Application of SEM and EDX in Studying Biomineralization in Plant Tissues
Honghua He
30. Freeze Stabilization and Cryopreparation Technique for Visualizing the Water Distribution in Woody Tissues by X-ray Imaging and Cryoscanning Electron Microscopy
Yasuhiro Utsumi and Yuzou Sano
31. Biological Applications of Energy-Filtered TEM
Martin Saunders and Jeremy A. Shaw
32. Secondary Ion Mass Spectrometry Imaging of Biological Cells and Tissues
Nicholas P. Lockyer
33. Elemental and Isotopic Imaging of Biological Samples using NanoSIMS
Matt R. Kilburn and Peta L. Clode
34. 3D Chemical Mapping: Application of Scanning Transmission (soft) X-ray Microscopy (STXM) in Combination with Angle-Scan Tomography in Bio-, Geo- and Environmental Science
Gregor Schmid and Martin Obst.
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