Electron microscopy : methods and protocols

著者

書誌事項

Electron microscopy : methods and protocols

（Methods in molecular biology / John M. Walker, series editor, 1117）（Springer protocols）

Humana Press, c2014

3rd ed. / edited by John Kuo

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注記

Previously published in 2007

Includes bibliographical references and index

内容説明・目次

内容説明

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.

目次

1. Conventional Specimen Preparation Techniques For Transmission Electron Microscopy of Cultured Cells John J. Bozzola 2. Microwave-assisted Processing and Embedding for Transmission Electron Microscopy Paul Webster 3. Processing Plant Tissues for Ultrastructural Study John Kuo 4. Staining Sectioned Biological Specimens for Transmission Electron Microscopy: Conventional and En Bloc Stains E. Ann Ellis 5. Metal Shadowing for Electron Microscopy Gregory M. Hendricks 6. Freeze Fracture and Freeze Etching Douglas E. Chandler and William P. Sharp 7. Conventional Specimen Preparation Techniques For Scanning Electron Microscopy of Biological Specimens John J. Bozzola 8. High-pressure Freezing: Current State and Future Prospects Andres Kaech and Urs Ziegler 9. Cryo-fixation by Self-pressurized Rapid Freezing Markus Grabenbauer, Hong-Mei Han, and Jan Huebinger 10. Cryo-Electron Microscopy of Vitreous Sections Petr Chlanda and Martin Sachse 11. Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine J. Robin Harris 12. Electron Microscopy of the Microtubule Cytoskeleton Assembly Vitro Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison 13. Cryosectioning Fixed and Cryoprotected Biological Material for Immmunocytochemistry Paul Webster and Alexandre Webster 14. Analysis of Specificity in Immunoelectron Microscopy Christian Hacker and John M. Lucocq 15. Cryo-Electron Microscopy of Membrane Proteins Kenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, and Henning Stahlberg 16. Tracking DNA and RNA Sequences at High Resolution Dusan Cmarko, Anna Ligasova, and Karel Koberna 17. Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy Yuri L. Lyubchenko, Alexander A. Gall, and Luda S. Shlyakhtenko 18. Biological Applications of Phase-Contrast Electron Microscopy Kuniaki Nagayama 19. Single Particle Cryo-Electron Microscopy And 3-D Reconstruction Of Viruses Fei Guo and Wen Jiang 20. Electron Tomography for Organelles, Cells and Tissues Wanzhong He and Yongning He 21. Correlative Light and Electron Microscopy: from Live Cell Dynamic to 3D Ultrastructure Coralie Spiegelhalter, Jocelyn F. Laporte, and Yannick Schwab 22. Nanometer-resolution Fluorescence Electron Microscopy (Nano-EM) in Cultured Cells Shigeki Watanabe, Martin Lehmann, Edward Hujber, Richard D. Fetter, Jackson Richards, Berit Soehl-Kielczynski, Annegret Felies, Christian Rosenmund, Jan Schmoranzer, and Erik M. Jorgensen 23. Correlative Fluorescence- and Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid Diana B. Peckys, Madeline J. Dukes, and Niels de Jonge 24. FIB-SEM Tomography in Biology Caroline Kizilyaprak 25. Correlative Light and Electron Microscopy using Immunolabeled Sections Heinz Schwarz and Bruno M. Humbel 26. Correlative 3D Imaging: CLSM and FIB-SEM Tomography Using High-Pressure Frozen, Freeze-Substituted Biological Samples Miriam S. Lucas, Maja Guenthert, Philippe Gasser, Falk Lucas and Roger Wepf 27. Three-dimensional Imaging of Adherent Cells using FIB-SEM and STEM Clarissa Villinger, Martin Schauflinger, Heiko Gregorius, Christine Kranz, Katharina Hoehn, Soufi Nafeey, Paul Walther 28. X-Ray Microanalysis in the Scanning Electron MicroscopeGodfried M. Roomans and Anca Dragomir 29. Application of SEM and EDX in Studying Biomineralization in Plant Tissues Honghua He 30. Freeze Stabilization and Cryopreparation Technique for Visualizing the Water Distribution in Woody Tissues by X-ray Imaging and Cryoscanning Electron Microscopy Yasuhiro Utsumi and Yuzou Sano 31. Biological Applications of Energy-Filtered TEM Martin Saunders and Jeremy A. Shaw 32. Secondary Ion Mass Spectrometry Imaging of Biological Cells and Tissues Nicholas P. Lockyer 33. Elemental and Isotopic Imaging of Biological Samples using NanoSIMS Matt R. Kilburn and Peta L. Clode 34. 3D Chemical Mapping: Application of Scanning Transmission (soft) X-ray Microscopy (STXM) in Combination with Angle-Scan Tomography in Bio-, Geo- and Environmental Science Gregor Schmid and Martin Obst.

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