Cytotoxic T-cells : methods and protocols
著者
書誌事項
Cytotoxic T-cells : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1186）（Springer protocols）
Humana Press, c2014
大学図書館所蔵 件 / 全2件
-
該当する所蔵館はありません
- すべての絞り込み条件を解除する
注記
Includes bibliographical references and index
内容説明・目次
内容説明
In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.
目次
1. Negative And Positive Separation Techniques For The Isolation Of Antigen-Specific Cd8+ T Cells From Blood And Tumor Tissue
Margherita Gigante, Sharon Natasha Cox, and Elena Ranieri
2. In Vitro\Ex Vivo Generation Of Cytotoxic T Lymphocytes
Margherita Gigante and Elena Ranieri
3. Phenotypic And Functional Characterization Of Cytotoxic T Lymphocytes By Flow Cytometry
Lulia Popescu, Matthew Pipeling, Jason Akulian, and John Mcdyer
4. Intracellular Signalling Of Ctls
Sharon Natasha Cox
5. Monitoring Antigen-Specific T Cell Responses Using Real-Time Pcr
Devin B. Lowe, Jennifer L. Taylor, And Walter J. Storkus
6. Ctl Elispot Assay
Elena Ranieri, Iulia Popescu, and Margherita Gigante
7. Methods Of Purification Of Ctl-Derived Exosomes
Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli
8. Characterization Of Ctl By Microscopy
Giuseppe Stefano Netti
9. Intravital Imaging Of Cytotoxic T Lymphocytes
Gaetano Faleo, Per-Olof Berggren, and Antonello Pileggi
10. The Microarray-Based Approach For The Analysis Of The Transcriptome
Matteo Accetturo, Paola Pontrelli, and Loreto Gesualdo
11. Mirnome Analysis Using Real Time Pcr
Paola Pontrelli, Matteo Accetturo, and Loreto Gesualdo
12. Proteomic Approaches By Seldi And Maldi-Tof/Ms For Ctl Analysis
Massimo Papale and Maria Teresa Rocchetti
13. Two-Dimensional Gel Electrophoresis Approach For Ctl Phosphoproteome Analysis
Maria Teresa Rocchetti, Massimo Papale, and Loreto Gesualdo
14. Targeting The Jak/Stat Pathway In Cytotoxic T Lymphocytes (Ctl) By Next Generation Sequencing (Ngs)
Maddalena Gigante, Sterpeta Diella, and Elena Ranieri
15. Experimental Model For Studying The Involvement Of Regulatory Cytotoxic T Cells In Bone Resorption
Giacomina Brunetti, Angela Oranger, Silvia Colucci, and Maria Grano
16. Ctl And Transplantation: Tissue In Vivo Characterization
Federica Rascio, Chiara Divella, and Giuseppe Grandaliano
「Nielsen BookData」 より
