Protein purification : principles and practice
著者
書誌事項
Protein purification : principles and practice
（Springer advanced texts in chemistry / Charles R. Cantor, editor）
Springer Science+Business Media, c2010
3rd ed
- タイトル別名
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Protein purification : principles and practice : with 165 figures
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注記
Includes bibliographical references (p. [356]-373) and index
内容説明・目次
内容説明
New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.
目次
- Prefaces
- 1. The Protein Purification Laboratory
- 2. Making an Extract
- 3. Analysis- Measurement of Protein and Enzyme Activity
- 4. Separation by Precipitation
- 5. Separation by Adsorption I: General Principles
- 6. Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents
- 7. Separation by Adsorption: Affinity Techniques
- 8. Separation in Solution
- 9. Purification of Special Types of Proteins
- 10. Small-Scale and Large-Scale Procedures
- 11. Analysis for Purity
- 12. Optimization of Procedures: Final Steps
- Appendix A-D
- References
- Index
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