Instrumental methods for the analysis and identification of bioactive molecules
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書誌事項
Instrumental methods for the analysis and identification of bioactive molecules
(ACS symposium series, 1185)
American Chemical Society , Distributed in print by Oxford University Press, c2014
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注記
"This ACS Symposium Series Book was evolved from the ACS symposium “Instrumental Methods for Analysis of Bioactive Molecules", sponsored by the Division of Agricultural and Food Chemistry at the 246th American Chemical Society National Meeting & Exposition in Indianapolis, Indiana, September 8-12, 2013."--Pref.
Includes bibliographical references and index
収録内容
- Influence of postharvest storage, processing, and extraction methods on the analysis of phenolic phytochemicals
- Innovative methods for the extraction and chromatographic analysis of honey bee products
- Separation and identification of cucurbitane-type triterpenoids from bitter melon
- Fast analysis of bioactive compounds by reverse phase liquid chromatography
- Chromatography combined with bioassays and other hyphenations : the direct link to the compound indicating the effect, identification by LC-MS and LC-NMR
- Standardization of analytical methodology and procedures for purity assessment of small molecules in drug discovery
- Biofunctional properties of melia azedarach extracts
- Characterization of sambucus nigra L. infusions using capillary electrophoresis and liquid chromatography-tandem mass spectrometry
- Flavonoid c-glycosides in citrus juices from southern Italy : distribution and influence on the antioxidant activity
- Characterization of pomegranate's health benefiting bioactive compounds, taste, color and post-harvest fruit quality by studying a wide collection of diverse accessions
- Application of HPLC-SPE-NMR in characterization of bioactive natural compounds
- Identification by GC, GC-MS, LC-GC and spectroscopic methods
- Headspace and solid-phase micro-extraction methods for the identification of volatile flavor compounds in citrus fruits
- GC and on-line LC-GC : useful tools for the qualitative and quantitative analysis of phytosterols and their esters
- Unraveling food aroma: methods for odor active compounds analysis
- Quantification of health-promoting compounds by quantitative 1HMNR spectroscopy
- Meat freshness : peroxynitrite's oxidative role, its natural scavengers, and new measuring tools
- Measurement of resistant starches in rat cecal contents using fourier transform infrared photoacoustic spectroscopy
- Metabolomic fingerprinting
- Metabolic fingerprinting of the lycopodiales species for chemotaxonomy and quality control
- New approaches in metabolic fingerprinting : improved extraction method and automatic reduction of NMR spectra to essential data
内容説明・目次
内容説明
The main purpose of this book is to discuss cutting-edge research techniques in the field of biologically active compounds derived from plants and foods, and in particular to describe advanced instrumental methods for the identification and quantification of these important compounds. Natural products are used worldwide to prevent and treat various diseases. The efficacy and safety of natural products require the development and application of reliable analytical
methods to regulate and control usage. Because of their complex chemical compositions, natural products often require separation techniques for reliable analysis of their constituents. This book provides an integrated approach to address the separation, identification, quantification and chemistry of
natural products for their analysis through various instrumental analyses.
This book is divided into four sections: 1) Sample Preparation, Separation and Identification 2) Identification by LC-MS and LC-NMR 3) Identification by GC, GC-MS, LC-GC and Spectroscopic Methods and 4). Metabolomic Fingerprinting. The first section covers sample preparation, extraction and rapid flash separation techniques, rapid LC analysis with bioassays and hyphenations to identify active constituents. The second section covers chromatographic separation by LC for active constituents,
identification by mass spectra, and LC-NMR for the characterization of specific constituents. The third section covers separation of volatile constituents by GC and GC-MS. In recent years, analysis of volatiles using headspace has become more sensitive and popular, as it does not alter the original
composition of volatile chemicals. The fourth section focuses on metabolic fingerprinting techniques. Metabolomics involves the study of small molecules from cells, plants, foods, tissues, organisms, or other biological tissues. Metabolic fingerprinting compares patterns, also called signatures or fingerprints of metabolites; these patterns change in response to external stimuli either in different plant varieties or species, or in response to adulteration, toxins, drug exposure, environmental
or genetic alterations.
This book comprehensively provides a current review that will be useful for scientists, researchers, teachers, and engineers in general, and is particularly useful for chemists, biochemists, chemical engineers, biochemical engineers, and others in chemistry-related fields.
目次
Preface
Sample Preparation, Separation, and Identification
1. Influence of Postharvest Storage, Processing, and Extraction Methods on the Analysis of Phenolic Phytochemicals
2. Innovative Methods for the Extraction and Chromatographic Analysis of Honey Bee Products
3. Separation and Identification of Cucurbitane-Type Triterpenoids from Bitter Melon
4. Fast Analysis of Bioactive Compounds by Reverse Phase Liquid Chromatography
5. Chromatography Combined with Bioassays and Other Hyphenations--The Direct Link to the Compound Indicating the Effect
Identification by LC-MS and LC-NMR
6. Standardization of Analytical Methodology and Procedures for Purity Assessment of Small Molecules in Drug Discovery
7. Biofunctional Properties of Melia azedarach Extracts
8. Characterization of Sambucus nigra L. Infusions Using Capillary Electrophoresis and Liquid Chromatography-Tandem Mass Spectrometry
9. Flavonoid C-glycosides in Citrus Juices from Southern Italy: Distribution and Influence on the Antioxidant Activity
10. Characterization of Pomegranate's Health Benefiting Bioactive Compounds, Taste, Color, and Post-Harvest Fruit Quality by Studying a Wide Collection of Diverse Accessions
11. Application of HPLC--SPE--NMR in Characterization of Bioactive Natural Compounds
Identification by GC, GC-MS, LC-GC, and Spectroscopic Methods
12. Headspace and Solid-Phase Microextraction Methods for the Identification of Volatile Flavor Compounds in Citrus Fruits
13. GC and On-line LC-GC: Useful Tools for the Qualitative and Quantitative Analysis of Phytosterols and Their Esters
14. Unraveling Food Aroma: Methods for Odor Active Compounds Analysis
15. Quantification of Health-Promoting Compounds by Quantitative 1HNMR Spectroscopy
16. Meat Freshness: Peroxynitrite's Oxidative Role, Its Natural Scavengers, and New Measuring Tools .
17. Measurement of Resistant Starches in Rat Cecal Contents using Fourier Transform Infrared Photoacoustic Spectroscopy
Metabolomic Fingerprinting
18. Metabolic Fingerprinting of the Lycopodiales Species for Chemotaxonomy and Quality Control
19. New Approaches in Metabolic Fingerprinting: Improved Extraction Method and Automatic Reduction of NMR Spectra to Essential Data
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