Plant mitochondria : methods and protocols

著者

    • Whelan, James
    • Murcha, Monika W.

書誌事項

Plant mitochondria : methods and protocols

edited by James Whelan, Monika W. Murcham

(Methods in molecular biology / John M. Walker, series editor, 1305)(Springer protocols)

Humana Press, c2015

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.

目次

1. Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa Monika W. Murcha and James Whelan 2. Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5' End Maturation Stefan Binder and Kristina Kuhn 3. Mitochondrial Run-On Transcription Assay Using Biotin Labeling Kristina Kuhn 4. In Vitro RNA Uptake Studies in Plant Mitochondria Szymon Kubiszewski-Jakubiak, Cyrille Megel, Elodie Ubrig, Thalia Salinas, Anne-Marie Duchene, and Laurence Marechal-Drouard 5. In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria Owen Duncan, Chris Carrie, Yan Wang, and Monika W. Murcha 6. Plant Mitochondrial Proteomics Nicolas L. Taylor and A. Harvey Millar 7. Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites Markus Hartl, Ann-Christine Koenig, and Iris Finkemeier 8. A Flowchart to Analyze Protease Activity in Plant Mitochondria Pedro F. Teixeira, Rui M. Branca, Beata Kmiec, and Elzbieta Glaser 9. Activity Measurements of Mitochondrial Enzymes in Native Gels Peter Schertl and Hans-Peter Braun 10. Activity Assay for Plant Mitochondrial Enzymes Shaobai Huang, Chun Pong Lee, and A. Harvey Millar 11. Analysis of Type II NAD(P)H Dehydrogenases Kathleen L. Soole and Chevaun A. Smith 12. Assessment of Respiration in Isolated Plant Mitochondria Using Clark Type Electrodes Richard P. Jacoby, A. Harvey Millar, and Nicolas L. Taylor 13. Micro-Respiration Measurements in Plants Yun Shin Sew, A. Harvey Millar, and Elke Stroeher 14. Improvements to Define Mitochondrial Metabolomics Using Non Aqueous Fractionation Richard Fly, James Lloyd, Stephan Krueger, Alisdair Fernie, and Margaretha J. van der Merwe 15. Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana Theresa J. Reape, Joanna Kacprzyk, Niall Brogan, Lee Sweetlove, and Paul F. McCabe 16. Imaging and Analysis of Mitochondrial Dynamics in Living Cells Sanjaya B. Ekanayake, Amr M. El Zawily, Gael Paszkiewicz, Aurelia Rolland, and David C. Logan 17. Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors Stephan Wagner, Thomas Nietzel, Isabel Aller, Alex Costa, Mark D. Fricker, Andreas J. Meyer, and Markus Schwarzlander 18. In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide Marina Cvetkovska and Greg C. Vanlerberghe 19. Databases and Informatics Resources For Analysis of Plant Mitochondria Reena Narsai 20. Expression and Crystallization of the Plant Alternative Oxidase Benjamin May, Catherine Elliott, Momi Iwata, Luke Young, Julia Shearman, Mary S. Albury, and Anthony L. Moore

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