Xylem : methods and protocols
Author(s)
Bibliographic Information
Xylem : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1544)(Springer protocols)
Humana Press, c2017
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical Xylem: Methods and Protocols, aims to ensure successful results in the further study of this vital field.
Table of Contents
Part I: DEVELOPMENT
1 Experimental and Theoretical Methods to Investigate Vascular Development in Plants
Norma Fabregas, Pau Formosa-Jordan, Marta Ibanes, and Ana I. Cano-Delgado
2 Strigolactone-Mediated Stimulation of Secondary Xylem Proliferation in Stems
Javier Agusti
3 Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues
Sacha Escamez, Benjamin Bollhoener, and Hannele Tuominen
4 Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis
Delphine Menard, Henrik Serk, Raphael Decou, and Edouard Pesquet
5 Tissue Culture for Xylem Differentiation with Arabidopsis Leaves
Masato Saito, Alif Meem Nurani, Yuki Kondo, and Hiroo Fukuda
6 VND6-Induced Xylem Cell Differentiation in Arabidopsis Cell Cultures
Oda Yoshida
Part II: IMAGING
7 Live Imaging of Developing Xylem in Planta
Raymond Wightman
8 Immunolocalization in Secondary Xylem of Populus
Suzanne Gerttula and Andrew Groover
9 Monitoring Vascular Regeneration and Xylem Connectivity in Arabidopsis Thaliana
Charles Melnyk
10 Vascular Morphodynamics during Secondary Growth
Pierre Barbier De Reuille and Laura Ragni
11 Improved Mps-PI Staining for Xylem Characterization
Elisabeth Truernit
12 Chemical Imaging of Xylem by Raman Microspectroscopy
Andras Gorzsas
13 Using Cellprofiler to Analyse and Quantify Vascular Morphology
Liam Campbell, Manoj Kumar, and Simon Turner
Part III: COMPOSITION
14 Lignin Analysis by HPLC and FTIR
Jorge Reyes-Rivera and Teresa Terrazas
15 Carbohydrate Composition Analysis in Xylem
Baocai Zhang and Yihua Zhou
16 Structural Analysis of Cell Wall Polysaccharides Using PACE
Jennifer C. Mortimer
17 Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy
Raphael Decou, Henrik Serk, Delphine Menard, and Edouard Pesquet
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