Xylem : methods and protocols

著者

    • de Lucas, Miguel
    • Etchells, J. Peter

書誌事項

Xylem : methods and protocols

edited by Miguel de Lucas, J. Peter Etchells

(Methods in molecular biology / John M. Walker, series editor, 1544)(Springer protocols)

Humana Press, c2017

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Xylem: Methods and Protocols, aims to ensure successful results in the further study of this vital field.

目次

Part I: DEVELOPMENT 1 Experimental and Theoretical Methods to Investigate Vascular Development in Plants Norma Fabregas, Pau Formosa-Jordan, Marta Ibanes, and Ana I. Cano-Delgado 2 Strigolactone-Mediated Stimulation of Secondary Xylem Proliferation in Stems Javier Agusti 3 Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues Sacha Escamez, Benjamin Bollhoener, and Hannele Tuominen 4 Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis Delphine Menard, Henrik Serk, Raphael Decou, and Edouard Pesquet 5 Tissue Culture for Xylem Differentiation with Arabidopsis Leaves Masato Saito, Alif Meem Nurani, Yuki Kondo, and Hiroo Fukuda 6 VND6-Induced Xylem Cell Differentiation in Arabidopsis Cell Cultures Oda Yoshida Part II: IMAGING 7 Live Imaging of Developing Xylem in Planta Raymond Wightman 8 Immunolocalization in Secondary Xylem of Populus Suzanne Gerttula and Andrew Groover 9 Monitoring Vascular Regeneration and Xylem Connectivity in Arabidopsis Thaliana Charles Melnyk 10 Vascular Morphodynamics during Secondary Growth Pierre Barbier De Reuille and Laura Ragni 11 Improved Mps-PI Staining for Xylem Characterization Elisabeth Truernit 12 Chemical Imaging of Xylem by Raman Microspectroscopy Andras Gorzsas 13 Using Cellprofiler to Analyse and Quantify Vascular Morphology Liam Campbell, Manoj Kumar, and Simon Turner Part III: COMPOSITION 14 Lignin Analysis by HPLC and FTIR Jorge Reyes-Rivera and Teresa Terrazas 15 Carbohydrate Composition Analysis in Xylem Baocai Zhang and Yihua Zhou 16 Structural Analysis of Cell Wall Polysaccharides Using PACE Jennifer C. Mortimer 17 Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy Raphael Decou, Henrik Serk, Delphine Menard, and Edouard Pesquet

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詳細情報

  • NII書誌ID(NCID)
    BB23147417
  • ISBN
    • 9781493967209
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    New York
  • ページ数/冊数
    x, 259 p.
  • 大きさ
    27 cm
  • 親書誌ID
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