Lysosomes : methods and protocols
Author(s)
Bibliographic Information
Lysosomes : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1594）（Springer protocols）
Humana Press : Springer Nature, c2017
- : hard
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"This Humana Press imprint is published by Springer Nature. The registered company is Springer Science+Business Media LLC"--T.p. verso
Includes bibliographical references and index
Description and Table of Contents
Description
This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.
Table of Contents
1. SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS
Melanie Thelen, Dominic Winter, Thomas Braulke, and Volkmar Gieselmann
2. Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics
Mesut Bilgin, Jesper Nylandsted, Marja Jaattela, and Kenji Maeda
3. Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins
Elmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, and Olga Vagin
4. Analyzing Lysosome Related Organelles By Electron Microscopy
Ilse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, And Graca Raposo
5. Microscopic Analysis of Lysosomal Membrane Permeabilization
Ana Maria Vilamill Giraldo, Karin OEllinger, and Vesa Loitto
6. Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets
Frederik W. Lund and Daniel Wustner
7. Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues
Mingguang Ren, Beibei Deng, Xiuqi Kong, Yonghe Tang, and Weiying Lin
8. Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery
Takanobu Otomo and Tamotsu Yoshimori
9. Delivery of Cargo to Lysosomes using Gneosomes
Kristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, and Yitzhak Tor
10. Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles
Camilla Loeoev and Anna Erlandsson
11. Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells
Ida Eriksson, Karin OEllinger, and Hanna Appelqvist
12. Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
Anna L. Soedergren and Sofia Ramstroem
13. Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes
Norma W. Andrews
14. Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates
Neil D. Rawlings
15. Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex
Giuseppina Di Fruscio, Sandro Banfi, Vincenzo Nigro, and Andrea Ballabio
16. Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms
Yoko Hashimoto
17. Methods for Determination of -Glycosidase, -Glycosidase, and -Galactosidase Activities in Dried Blood Spot Samples
Eser Yildirim Sozmen and Ebru Demirel Sezer
18. Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi
Jyotsna Vermaa, Sunita Bijarnia-Mahaya, and Ishwar C. Vermaa
19. Lysosomal Biology in Cancer
Colin Fennelly and Ravi Amaravadi
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