Lysosomes : methods and protocols

著者

• Öllinger, Karin
• Appelqvist, Hanna

書誌事項

Lysosomes : methods and protocols

edited by Karin Öllinger, Hanna Appelqvist

（Methods in molecular biology / John M. Walker, series editor, 1594）（Springer protocols）

Humana Press : Springer Nature, c2017

• : hard

注記

"This Humana Press imprint is published by Springer Nature. The registered company is Springer Science+Business Media LLC"--T.p. verso

Includes bibliographical references and index

内容説明・目次

内容説明

This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.

目次

1. SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS Melanie Thelen, Dominic Winter, Thomas Braulke, and Volkmar Gieselmann 2. Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics Mesut Bilgin, Jesper Nylandsted, Marja Jaattela, and Kenji Maeda 3. Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins Elmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, and Olga Vagin 4. Analyzing Lysosome Related Organelles By Electron Microscopy Ilse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, And Graca Raposo 5. Microscopic Analysis of Lysosomal Membrane Permeabilization Ana Maria Vilamill Giraldo, Karin OEllinger, and Vesa Loitto 6. Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets Frederik W. Lund and Daniel Wustner 7. Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues Mingguang Ren, Beibei Deng, Xiuqi Kong, Yonghe Tang, and Weiying Lin 8. Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery Takanobu Otomo and Tamotsu Yoshimori 9. Delivery of Cargo to Lysosomes using Gneosomes Kristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, and Yitzhak Tor 10. Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles Camilla Loeoev and Anna Erlandsson 11. Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells Ida Eriksson, Karin OEllinger, and Hanna Appelqvist 12. Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry Anna L. Soedergren and Sofia Ramstroem 13. Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes Norma W. Andrews 14. Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates Neil D. Rawlings 15. Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex Giuseppina Di Fruscio, Sandro Banfi, Vincenzo Nigro, and Andrea Ballabio 16. Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms Yoko Hashimoto 17. Methods for Determination of -Glycosidase, -Glycosidase, and -Galactosidase Activities in Dried Blood Spot Samples Eser Yildirim Sozmen and Ebru Demirel Sezer 18. Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi Jyotsna Vermaa, Sunita Bijarnia-Mahaya, and Ishwar C. Vermaa 19. Lysosomal Biology in Cancer Colin Fennelly and Ravi Amaravadi

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詳細情報

• NII書誌ID(NCID): BB24315211
• ISBN:
  • 9781493969326
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiii, 311 p.
• 大きさ: 27 cm
• 親書誌ID:
  • BA10794673
  • BA88873632