T-cell trafficking : methods and protocols

著者

    • Rainger, George Edward
    • Mcgettrick, Helen M.

書誌事項

T-cell trafficking : methods and protocols

edited by George Edward Rainger, Helen M. Mcgettrick

（Methods in molecular biology / John M. Walker, series editor, v. 1591）（Springer protocols）

Humana Press, c2017

2nd ed

  • : hbk.

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during 'physiological' resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.

目次

1. Introduction to Homeostatic Migration Mark C. Coles 2. Analysis of Thymocyte Migration, Cellular Interactions, and Activation by Multi-Photon Fluorescence Microscopy of Live Thymic Slices Jessica N. Lancaster and Lauren I. R. Ehrlich 3. Visualising and Tracking T Cell Motility in vivo Robert A Benson, James M Brewer, and Paul Garside 4. Graph Theory-based Analysis of the Lymph Node Fibroblastic Reticular Cell Network Mario Novkovic, Lucas Onder, Gennady Bocharov, and Burkhard Ludewig 5. Visualizing Endogenous Effector T Cell Egress from the Lymph Nodes Manisha Menon, Alexandre P Benechet, and Kamal M Khanna 6. Introduction: T cell Trafficking in Inflammation and Immunity Myriam Chimen, Bonita H. R. Apta, and Helen M. McGettrick 7. Leukocyte Adhesion under Haemodynamic Flow Conditions Charlotte Lawson, Marlene Rose, and Sabine Wolf 8. Endocrine Regulation of Lymphocyte Trafficking In Vitro Bonita H. R. Apta, Myriam Chimen, and Helen M. McGettrick 9. Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells Helen M. McGettrick, Lewis S.C. Ward, G. Ed Rainger, and Gerard B. Nash 10. Monitoring RhoGTPase Activity in Leukocytes using Classic "pull-down" Assays Marouan Zarrouk, David Killock, Izajur Rahman, Jessica Davies, and Aleksandar Ivetic 11. Utilising Lentiviral Gene Transfer in Primary Endothelial Cells to Assess Lymphocyte-Endothelial Interactions Jasmeet S. Reyat, Michael G. Tomlinson, and Peter J. Noy 12. Introduction to Lymphocyte Trafficking in Disease Patricia F. Lalor and Elizabeth Hepburn 13. Using Ex-Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking Ben Wiggins, Zania Stamataki, and Patricia F. Lalor 14. In vitro and Ex Vivo Models to Study T Cell Migration through the Human Liver Parenchyma Benjamin G. Wiggins, Konstantinos Aliazis, and Scott P. Davies 15. Monitoring Migration of Activated T Cells to Antigen-rich Non-lymphoid Tissue Eleanor Jayne Ward, Hongmei Fu, and Federica Marelli Berg 16. Tissue Digestion for Stromal Cell and Leukocyte Isolation Saba Nayar, Joana Campos, Nathalie Steinthal, and Francesca Barone 17. T-cell Response in the Lung Following Influenza Virus Infection Robert A. Benson, Jennifer C. Lawton, and Megan K.L. MacLeod

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