Biosafety assessment of probiotic potential
Author(s)
Bibliographic Information
Biosafety assessment of probiotic potential
（Methods and protocols in food science）
Springer, 2022
- hbk.
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Description and Table of Contents
Description
This volume provides methods on procedures for assessing the biosafety aspects of probiotics. Chapters are divided into five parts detailing in vitro biosafety assessment, biogenic amine production, D-lactic acid production, toxin production, production of various enzymes, determination of toxicity, mutagenicity, virulence genes, capsule formation, hemolytic activity, DNAse activity, bile salt deconjugation, antibiotic resistance, antibiotic resistance gene transfer, mucin degradation, platelet aggregation, and in vivo biosafety assessment of probiotics including determination of infectivity, reproductive and developmental toxicity, and evaluation of immunological parameters in animal models.
Authoritative and cutting-edge, Biosafety Assessment of Probiotic Potential aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
Table of Contents
Part I: In vitro Biosafety Assessment of Probiotics
1. Determination of biogenic Amine Production
Shilpika Pandey, Archana Chaudhari, and Mitesh Kumar Dwivedi
2. Determination of Gelatinases,Glycosidases, and Enolase production
Archana Chaudhari, Shilpika Pandey, and Mitesh Kumar Dwivedi
3. Determination of -Glucuronidase Production
Dixita Panchal, Vrutika Lad, Meonis Pithawala, and Natarajan Amaresan
4. Determination of Nitroreductase Production
Dixita Panchal, Vrutika Lad, Meonis Pithawala, and Natarajan Amaresan
5. Determination of Azoreductase production
Vrutika Lad, Dixita Panchal, Meonis Pithawala, and Natarajan Amaresan
6. Determination of Hemolytic activity
Vrutika Lad, Dixita Panchal, Meonis Pithawala, Mitesh Kumar Dwivedi, and Natarajan Amaresan
7. Determination of DNAse Activity
Archana Chaudhari, Ruma Raghuvanshi, and Mitesh Kumar Dwivedi
8. Determination of Bile Salts Deconjugation
Hemant Borase, Satish Patil2, Mitesh Kumar Dwivedi, and Ramar Krishnamurthy
9. Determination of D-lactic Acid Production
Shilpika Pandey, Archana Chaudhari, Mitesh Kumar Dwivedi
10. Determination of Antibiotic Resistance
M. Veerapagu, K.R. Jeya, Ashraf Khalifa, and A. Sankaranarayanan
11. Determination of Antibiotic Resistance Gene Transfer
K.R. Jeya, Ashraf Khalifa, M. Veerapagu, and A. Sankaranarayanan
12. Determination of Toxin Production
Urjita V. Sheth
13. Detection of Toxin Genes by PCR Based Methods
Urjita V. Sheth
14. Determination of Presence of Plasmids and Virulence and Structural Genes
Teresa Semedo-Lemsaddek and Maria Joao Fraqueza
15. Determination of Toxicity through Cytotoxicity Assays
Urjita V. Sheth
16. In vitro Evaluation of the Nitric Oxide Pathway
Hemant Borase, Satish Patil, Dhruti Amin, and Mitesh Kumar Dwivedi
17. Assessment of Capsule Formation
Rushabh Shah, Natarajan Amaresan, and Mitesh Kumar Dwivedi
18. Assessment of Platelet aggregation
Karthick Raja Arulprakasam and Dhanasekaran Dharumadurai
19. Assessment of Fibrinogen and Fibronectin Binding Activity
Suresh Singh Yadav and Pramod Kumar
20. Assessment of Probiotics Adhesion to Mammalian Cells
Guhanraj Radhamanalan and Dhanasekaran Dharumadurai
21. Assessment of Mutagenicity
K.R. Jeya, Ashraf Khalifa, M. Veerapagu, and A. Sankaranarayanan
22. Assessment of Induction and Destruction of Thrombi
M. Veerapagu, K.R. Jeya, and A. Sankaranarayanan
23. Assessment of degradation of mucin
Ruma Raghuvanshi, Archana Chaudhari, Mitesh Kumar Dwivedi
Part II: In vivo Biosafety Assessment of Probiotics
24. Evaluation of General Health Status of the Animals during the in-Life Phase
Hafiz Arbab Sakandar, Qaiser Farid Khan, Zainab Tahir, Hamid Majeed, Nabaa Azhar, and Zaib un Nisa
25. Assessment of Bacterial Translocation Through Blood Cultures
Li Hui Ang, Premmala Rangasamy, Wallace Jeng Yang Chee, Shu Yih Chew, and Leslie Thian Lung Than
26. Determination of Splenic weight index and Weight-to-length ratio
Firdosh Shah and Mitesh Kumar Dwivedi
27. Determination of Total liver glutathione and plasma malondialdehyde concentrations
Firdosh Shah and Mitesh Kumar Dwivedi
28. Determination of Serum Lactate and Fecal Calprotectin for Assessing The Intestinal Inflammation
Firdosh Shah and Mitesh Kumar Dwivedi
Part III: Measuring infectivity in animal models
29. In vivo Evaluation of Adhesion properties of Probiotics
Nabendu Debnath, Pooja Yadav, Ashish Tyagi, and Ashok Kumar Yadav
30. Determination of Streptomyces Probiotics oral administration in broiler chicken
Latha Selvanathan, Thirumagal Thirugnanam, Vinothini Gopal and Dhanasekaran Dharumadurai
31. Determination of infectivity of probiotics using animal models
Shabari Girish and Lokesh Ravi
32. Determination of Infectivity of Probiotics using Immunosuppressed Hosts
Sumana Chatterjee and Tamalika Chakraborty
Part IV: Measuring Reproductive and Developmental toxicity in animal models
33. Assessment of Reproductive toxicity
Firdosh Shah and Mitesh Kumar Dwivedi
34. Assessment of Developmental toxicity in Zebrafish model
Tamalika Chakraborty, Sumana Roy, Dipanjan Mandal, and Jeenatara Begum
Part V: Measuring Immunological Parameters in animal models for Safety
35. Assessment of Inflammation in Animal Models (Macroscopic Or Histological Inflammation In The Ileum Or In The Colon)
Sumana Roy, Tamalika Chakraborty, Prerona Saha, and Sriparna Kundu Sen
36. Assessment of inflammation in animal models (Quantification of TNFA, IFNG, IL4, and IL10 mRNAs by Real-time PCR)
Sachin Kumar, Rashmi H.M., and Brijesh Kumar
37. Assessment of inflammation in animal models (Quantification of TNF- , IFN- , IL-4, & IL-10 proteins by ELISA)
Siti Nor Hikmah Abdul Rasid, Angela Boahen, Shu Yih Chew, Shalini Vellasamy, and Leslie Thian Lung Than
38. Detection of Myeloperoxidase activity by Enzyme Linked Immunosorbent Assay
Pooja Yadav, Nabendu Debnath, Ashish Tyagi, and Ashok Kumar Yadav
39. Assessment of Bacterial Translocation Through Mesenteric Lymph Nodes [MLN] and Spleen Cultures
Fida Fathima and Rekha P.D.
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