Environmental responses in plants : methods and protocols
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Bibliographic Information
Environmental responses in plants : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2494）（Springer protocols）
Humana Press, c2022
2nd ed
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Includes bibliographical references and index
Description and Table of Contents
Description
This second edition describes up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Chapters are divided into four sections detailing protocols to investigate key biological processes underlying plant environmental responses in the model organisms Arabidopsis thaliana and Physcomitrella patens as well as in different crop species. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Environmental Responses in Plants, Second Edition aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
Table of Contents
PART I Tropisms and OTHER Developmental responses
1 Analysis of Plant Root Gravitropism
Richard Barker, Sarah Johns, Ralph Trane, and Simon Gilroy
2 Hydrotropism: Analysis of the Root Response to a Moisture Gradient
Irene Garcia-Maquilon, Jorge Lozano-Juste, Abdulwahed F. Alrefaei, and Pedro L. Rodriguez
3 Evaluating Root Mechanosensing Response in Rice
Tiago F. Lourenco, Andre M. Cordeiro, Joao Frazao, Nelson J.M. Saibo, and M. Margarida Oliveira
4 Physiological Analysis of Phototropic Responses to Blue and Red Light in Arabidopsis
Mathias Zeidler
5 Shoot Branching Phenotyping in Arabidopsis and Tomato
Ana Confraria, Aitor Munoz-Gasca, Liliana Ferreira, Elena Baena-Gonzalez, and Pilar Cubas
6 Ribo-Seq Method to Study Genome-Wide Translational Regulation in Plants
Hao Chen, Jose M. Alonso, and Anna N. Stepanova
PART II Photoperiodism and Circadian Rythms
7 Assessing Flowering Time Under Different Photoperiods
Jesus Praena, Elizabeth van Veen, Rossana Henriques, and Reyes Benlloch
8 Analysis of Phytochrome-Dependent Seed Germination in Arabidopsis
Mathias Zeidler
9 Rhythmic Leaf and Cotyledon Movement Analysis
Ping Lou, Kathleen Greenham, and C. Robertson McClung
10 Assessing Plant Pigment Regulation in Circadian Experiments
Raquel Esteban, Victor Resco de Dios, and Jose Ignacio Garcia-Plazaola
11 Chemical perturbation of Chloroplast Ca2+ Dynamics in Arabidopsis thaliana Suspension Cell Cultures and Seedlings
Panfeng Yao, Steffen Vanneste, Lorella Navazio, Frank Van Breusegem, and Simon Stael
PART III Abiotic Stress Responses
12 Screening for Abiotic Stress Response in Rice
Telma Fernandes, Fredilson Melo, Maria Beatriz Vieira, Tiago F. Lourenco, Chiara Pucciariello, Nelson J. M. Saibo, Isabel A. Abreu, and M. Margarida Oliveira
13 Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion
Guiomar Martin and Paula Duque
14 Assessing Postgermination Development in Arabidopsis thaliana under Abiotic Stress
Rui Albuquerque-Martins, Alba Rodriguez Diez, Dora Szakonyi, and Paula Duque
15 Assessing Abscisic Acid-Mediated Changes in Stomatal Aperture Through High-Quality Leaf Impressions
Alba Rodriguez Diez, Paula Duque, and Rossana Henriques
16 Evaluation of the Anti-Transpirant Activity of ABA-Receptor Agonists in Monocot and Eudicot Plants
Daniel Franco-Aragon, Irene Garcia-Maquilon, Alfredo Manicardi, Pedro L. Rodriguez, and Jorge Lozano-Juste
17 Quantifying Phytohormones In Vivo with FRET Biosensors and the FRETENATOR Analysis Toolset
James H. Rowe, Annalisa Rizza, and Alexander M. Jones
18 Optical Imaging Resources for Crop Phenotyping and Stress Detection
Phatchareeya Waiphara, Cyril Bourgenot, Lindsey J Compton, and Ankush Prashar
PART IV Plant Interactions WITH OTHER ORGANISMS
19 Analysis of Innate Immune Responses Against Pathogenic Bacteria in Arabidopsis, Tomato and Barley Marion Wenig, Kornelia Bauer, Miriam Lenk, and A. Corina Vlot
20 Performing Infection Assays of Sporisorium reilianum f. sp. Zeae in Maize
Mamoona Khan and Armin Djamei
21 A Method to Measure the Damage Caused by Cell-Sucking Herbivores
Andre F. Mira, Luis Marques, Sara Magalhaes, and Leonor R. Rodrigues
22 RNA Isolation from Nematode-induced Feeding Sites in Arabidopsis Roots Using Laser Capture Microdissection
Muhammad Shahzad Anjam, Shahid Siddique, and Peter Marhavy
23 High-Throughput Profiling of Root-Associated Microbial Communities
Felix Getzke and Stephane Hacquard
24 Analysis of Immunity-Related Oxidative Bursts by a Luminol-Based Assay
Bushra Saeed and Marco Trujillo
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