Environmental responses in plants : methods and protocols

著者

    • Duque, Paula
    • Szakonyi, Dóra

書誌事項

Environmental responses in plants : methods and protocols

edited by Paula Duque, Dóra Szakonyi

(Methods in molecular biology / John M. Walker, series editor, 2494)(Springer protocols)

Humana Press, c2022

2nd ed

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This second edition describes up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Chapters are divided into four sections detailing protocols to investigate key biological processes underlying plant environmental responses in the model organisms Arabidopsis thaliana and Physcomitrella patens as well as in different crop species. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Environmental Responses in Plants, Second Edition aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

目次

PART I Tropisms and OTHER Developmental responses 1 Analysis of Plant Root Gravitropism Richard Barker, Sarah Johns, Ralph Trane, and Simon Gilroy 2 Hydrotropism: Analysis of the Root Response to a Moisture Gradient Irene Garcia-Maquilon, Jorge Lozano-Juste, Abdulwahed F. Alrefaei, and Pedro L. Rodriguez 3 Evaluating Root Mechanosensing Response in Rice Tiago F. Lourenco, Andre M. Cordeiro, Joao Frazao, Nelson J.M. Saibo, and M. Margarida Oliveira 4 Physiological Analysis of Phototropic Responses to Blue and Red Light in Arabidopsis Mathias Zeidler 5 Shoot Branching Phenotyping in Arabidopsis and Tomato Ana Confraria, Aitor Munoz-Gasca, Liliana Ferreira, Elena Baena-Gonzalez, and Pilar Cubas 6 Ribo-Seq Method to Study Genome-Wide Translational Regulation in Plants Hao Chen, Jose M. Alonso, and Anna N. Stepanova PART II Photoperiodism and Circadian Rythms 7 Assessing Flowering Time Under Different Photoperiods Jesus Praena, Elizabeth van Veen, Rossana Henriques, and Reyes Benlloch 8 Analysis of Phytochrome-Dependent Seed Germination in Arabidopsis Mathias Zeidler 9 Rhythmic Leaf and Cotyledon Movement Analysis Ping Lou, Kathleen Greenham, and C. Robertson McClung 10 Assessing Plant Pigment Regulation in Circadian Experiments Raquel Esteban, Victor Resco de Dios, and Jose Ignacio Garcia-Plazaola 11 Chemical perturbation of Chloroplast Ca2+ Dynamics in Arabidopsis thaliana Suspension Cell Cultures and Seedlings Panfeng Yao, Steffen Vanneste, Lorella Navazio, Frank Van Breusegem, and Simon Stael PART III Abiotic Stress Responses 12 Screening for Abiotic Stress Response in Rice Telma Fernandes, Fredilson Melo, Maria Beatriz Vieira, Tiago F. Lourenco, Chiara Pucciariello, Nelson J. M. Saibo, Isabel A. Abreu, and M. Margarida Oliveira 13 Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion Guiomar Martin and Paula Duque 14 Assessing Postgermination Development in Arabidopsis thaliana under Abiotic Stress Rui Albuquerque-Martins, Alba Rodriguez Diez, Dora Szakonyi, and Paula Duque 15 Assessing Abscisic Acid-Mediated Changes in Stomatal Aperture Through High-Quality Leaf Impressions Alba Rodriguez Diez, Paula Duque, and Rossana Henriques 16 Evaluation of the Anti-Transpirant Activity of ABA-Receptor Agonists in Monocot and Eudicot Plants Daniel Franco-Aragon, Irene Garcia-Maquilon, Alfredo Manicardi, Pedro L. Rodriguez, and Jorge Lozano-Juste 17 Quantifying Phytohormones In Vivo with FRET Biosensors and the FRETENATOR Analysis Toolset James H. Rowe, Annalisa Rizza, and Alexander M. Jones 18 Optical Imaging Resources for Crop Phenotyping and Stress Detection Phatchareeya Waiphara, Cyril Bourgenot, Lindsey J Compton, and Ankush Prashar PART IV Plant Interactions WITH OTHER ORGANISMS 19 Analysis of Innate Immune Responses Against Pathogenic Bacteria in Arabidopsis, Tomato and Barley Marion Wenig, Kornelia Bauer, Miriam Lenk, and A. Corina Vlot 20 Performing Infection Assays of Sporisorium reilianum f. sp. Zeae in Maize Mamoona Khan and Armin Djamei 21 A Method to Measure the Damage Caused by Cell-Sucking Herbivores Andre F. Mira, Luis Marques, Sara Magalhaes, and Leonor R. Rodrigues 22 RNA Isolation from Nematode-induced Feeding Sites in Arabidopsis Roots Using Laser Capture Microdissection Muhammad Shahzad Anjam, Shahid Siddique, and Peter Marhavy 23 High-Throughput Profiling of Root-Associated Microbial Communities Felix Getzke and Stephane Hacquard 24 Analysis of Immunity-Related Oxidative Bursts by a Luminol-Based Assay Bushra Saeed and Marco Trujillo

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詳細情報

  • NII書誌ID(NCID)
    BC17077256
  • ISBN
    • 9781071622964
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    New York
  • ページ数/冊数
    xiv, 349 p.
  • 大きさ
    27 cm
  • 分類
  • 件名
  • 親書誌ID
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