Recombinant DNA

著者

• Wu, Ray
• Grossman, Lawrence
• Moldave, Kivie

書誌事項

Recombinant DNA

edited by Ray Wu

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 68, 100, 101, 153-155, 216-218）

Academic Press, c1979-

• [pt. A]
• pt. B
• pt. C
• pt. D
• pt. E
• pt. F
• pt. G
• pt. H
• pt. I

注記

Pt. B-C edited by Ray Wu, Lawrence Grossman, Kivie Moldave. pt. D-E edited by Ray Wu, Lawrence Grossman. pt. F-I edited by Ray Wu

Includes bibliographical references and indexes

Place of publication varies: 1955-1983 New York, 1984-1986 Orlando, 1987- San Diego

内容説明・目次

巻冊次

[pt. A] ISBN 9780121819682

内容説明

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

巻冊次

pt. B ISBN 9780121820008

内容説明

FROM THE PREFACE: Exciting new developments in recombinant DNA research allow the isolation and amplification of specific genes or DNA segments from almost any living organism. These new developments have revolutionized our approaches to solving complex biological problems and have opened up new possibilities for producing new and better products in the areas of health, agriculture, and industry. Volumes 100 and 101 supplement Volumes 65 and 68 of Methods in Enzymology. During the last three years, many new or improved methods on recombinant DNA or nucleic acids have appeared, and they are included in these two volumes. Volume 100 covers the use of enzymes in recombinant DNA research, enzymes affecting the gross morphology of DNA, proteins with specialized functions acting at specific loci, new methods for DNA isolation, hybridization, and cloning, analytical methods for gene products, and mutagenesis: in vitro and in vivo. Volume 101 includes sections on new vectors for cloning genes, cloning of genes into yeast cells, and systems for monitoring cloned gene expression.

目次

• (Section Headings): Use of Enzymes in Recombinant DNA Research. Enzymes Affecting the Gross Morphology of DNA: Topoisomerases Type I
• Topoisomerases Type II. Proteins with Specialized Functions Acting at Specific Loci. New Methods for DNA Isolation, Hybridization, and Cloning. Analytical Methods for Gene Products. Mutagenesis: In Vitro and in Vivo. Author Index. Subject Index.

巻冊次

pt. C ISBN 9780121820015

内容説明

From the PrefaceExciting new developments in recombinant DNA research allow the isolation and amplification of specific genes or DNA segments from almost any living organism. These new developments have revolutionized our approaches to solving complex biological problems and have opened up new possibilities for producing new and better products in the areas of health, agriculture, and industry.Volumes 100 and 101 supplement Volumes 65 and 68 of Methods in Enzymology. During the last three years, many new or improved methods on recombinant DNA or nucleic acids have appeared, and they are included in these two volumes. Volume 100 covers the use of enzymes in recombinant DNA research, enzymes affecting the gross morphology of DNA, proteins with specialized functions acting at specific loci, new methods for DNA isolation, hybridization, and cloning, analytical methods for gene products, and mutagenesis: in vitro and in vivo. Volume 101 includes sections on new vectors for cloning genes, cloning of genes into yeast cells, and systems for monitoring cloned gene expression.

目次

New Vectors for Cloning Genes. Cloning of Genes into Yeast Cells. Systems for Monitoring Cloned Gene Expression: Intact Cell Systems Introduction of Genes into Mammalian Cells Cell-Free Systems Transcription Cell-Free Systems Translation. Author Index. Subject Index.

巻冊次

pt. D ISBN 9780121820541

内容説明

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

目次

Vectors for Cloning DNA:Production of Single-Stranded Plasmid DNA.pKUN, Vectors for the Separate Production of Both DNA Strands of Recombinant Plasmids.Restriction Site Bank Vectors for Cloning in Gram-Negative Bacteria and Yeast.Plasmids for the Selection and Analysis of Prokaryotic Promoters.A *gl DNA Protocol Based on Purification of Phage on DEAE-Cellulose.Double cos Site Vectors: Simplified Cosmid Cloning.Improved in Vitro Packaging of *gl DNA.*gl Phage Vectors--Embl Series.Plasmid and Phage Vectors for Gene Cloning and Analysis in Streptomyces.Cosmid Shuttle Vectors for Cloning and Analysis of Streptomyces DNA.Host-Vector Systems for Gene Cloning in Cyanobacteria.Genetic Engineering of the Cyanobacterial Chromosome.Conjugal Transfer of Plasmids to Cyanobacteria.Site-Directed Chromosomal Rearrangements in Yeast.Improved Vectors for Plant Transformation: Expression Cassette Vectors and New Selectable Markers.Vectors for Cloning in Plant Cells.Binary Ti Vectors for Plant Transformation and Promoter Analysis.Detection for Monocot Transformation via Agrobacterium tumefaciens.Direct Gene Transfer to Protoplasts of Dicotyledonous and Monocotyledonous Plants by a Number of Methods, Including Electroporation.Uptake of DNA and RNA into Cells Mediated by Electroporation.Electroporation of DNA and RNA into Plant Protoplasts.Cloning Vectors of Mitochondrial Origin for Eukaryotes.Vectors for Expression of Cloned Genes:Short Homopeptide Leader Sequences Enhanced Production of Human Proinsulin in Escherichia coli.Expression of Bovine Growth Hormone Derivatives in Escherichia coli and the Use of the Derivatives to Produce Natural Sequence Growth Hormone by Cathepsin C Cleavage.Expression of Eukaryotic Genes in Escherichia coli with a Synthetic Two-Cistron System.Expression of Heterologous Unfused Protein in Eshcerichia coli.Directing Ribosomes to a Single mRNA Species: A Method to Study Ribosomal RNA Mutations and Their Effects on Translation of a Single Messenger in Escherichia coli.New Expression Vectors for Identifying and Testing Signal Structures for Initiation and Termination of Transcription.Synthesis and Sequence-Specific Proteolysis of Hybrid Proteins Produced in Escherichia coli.Expression Plasmid Containing the *gl PL Promoter and cI857 Repressor.Expression and Secretion of Foreign Proteins in Escherichia coli.Engineering for Protein Secretion in Gram-Positive Bacteria. Expression and Secretion Vectors for Yeast.Vaccinia Virus as an Expression Vector.Author Index.Subject Index.

巻冊次

pt. E ISBN 9780121820558

内容説明

Recombinant DNA methods are powerful, revolutionary techniques for at least two reasons. First, they allow the isolation of single genes in large amounts from a pool of thousands or millions of genes. Second, the isolated genes or their regulatory regions can be modified at will and re-introduced into cells for expression at the RNA or protein levels. These attributes help to solve complex biological problems and to produce new and better products in the areas of health, agriculture and industry.Both volume 154 and 155 include the descriptions of several useful new restriction enzymes and rapid methods for DNA sequence analysis, along with a number of other useful methods.

目次

• Methods for Cloning cDNA. H. Okayama, M. Kawaichi, M. Brownstein, F. Lee, T. Yokota, and K. Arai, High-Efficiency Cloning of Full-Length cDNA
• Construction and Screening of cDNA Expression Libraries for Mammalian Cells. G. Heidecker and J. Messing, A Method for Cloning Full-Length cDNA in Plasmid Vectors. D.C. Alexander, An Efficient Vector*b1Primer cDNA Cloning System. C. Coleclough, Use of Primer*b1Restriction End Adapters in cDNA Cloning. Identification of Cloned Genes and Mapping of Genes. A.A. Reyes and R.B. Wallace, Mapping of RNA Using S1 Nuclease and Synthetic Oligonucleotides. C.G. Miyada and R.B. Wallace, Oligonucleotide Hybridization Techniques. M. Snyder, D. Sweetser, R.A. Young, and R.W. Davis, *glgt 11: Gene Isolation with Antibody Probes and Other Applications. M.R. Gray, G.P. Mazzara, P. Reddy, M. Rosbash, Searching for Clones with Open Reading Frames. G.M. Weinstock, Use of Open Reading Frame Expression Vectors. J.D. Boeke, J. Trueheart, G. Natsoulis, and G.R. Fink, 5-Flouroorotic Acid as a Selective Agent in Yeast Molecular Genetics. F.J. de Bruijn, Transposon Tn5 Mutagenesis to Map Genes. J.D. Noti, M.N. Jagadish, and A.A. Szalay, Site-Directed Tn5 and Transplacement Mutagenesis: Methods to Identify Symbiotic Nitrogen Fixation Genes in Slow-Growing Rhizobium. Chemical Synthesis and Analysis of Oligodeoxynucleotides. R. Frank, A. Meyerhans, K. Schwellnus, and H. Blvcker, Simultaneous Synthesis and Biological Applications of DNA Fragments: An Efficient and Complete Methodology. H.W. DjurhuusYMatthes, A. Staub, and P. Chambon, The Segmented Paper Method: DNA Synthesis and Mutagenesis by Rapid Microscale "Shotgun Gene Synthesis". M.H. Caruthers, A.D. Barone, S.L. Beaucage, D.R. Dodds, E.F. Fisher, L.J. McBride, M. Matteucci, Z. Stabinsky, and J.-Y. Tang, Chemical Synthesis of Deoxyoligonucleotides by the Phosphoramidite Method. S.J. Horvath, J.R. Firca, T. Hunkapiller, M.W. Hunkapiller, and L. Hood, An Automated DNA Synthesizer Employing Deoxynucleoside 3*b7-Phosphoramidites. Site-Specific Mutagenesis and Protein Engineering. M.J. Zoller, Oliconucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template. W. Kramer and H.-J. Fritz, Oligonucleotide-Directed Construction of Mutations via Gapped Duplex DNA. T.A. Kunkel, J.D. Roberts, and R.A. Zakour, Rapid and Efficient Site-Specific Mutagenesis without Phenotypic Selection. P. Carter, Improved Oligonucleotide-Directed Mutagenesis Using M13 Vectors. D.F. Mark, A. Wang, and C. Levenson, Site-Specific Mutagenesis to Modify the Human Tumor Necrosis Factor Gene. R. Pine and P.C. Huang, An Improved Method to Obtain a Large Number of Mutants in a Defined Region of DNA. P. Kollman and W.F. van Gunsteren, Molecular Mechanics and Dynamics in Protein Design. S. Pongor, The Use of Structural Profiles and Parametric Sequence Comparison in the Rational Design of Polypeptides. J.W. Taylor and E.T. Kaiser, Structure*b1Function Analysis of Proteins through the Design, Synthesis, and Study of Peptide Models. C.R. Matthews, Effect of Point Mutations on the Folding of Globular Proteins. T. Alber and B.W. Matthews, Structure and Thermal Stability of Phage T4 Lysozyme. Author Index. Subject Index.

巻冊次

pt. F ISBN 9780121820565

内容説明

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

目次

C.D. O'Connor, J.N.B. Walker, and J.R. Saunders, RsrII: A Restriction Endonuclease with a Heptanucleotide Recognition Sequence. B.-Q. Qiang and I. Schildkraut, NotI and SfiI: Restricton Endonucleases with Octanucleotide Recognition Sequences. M. McClelland, Site-Specific Cleavage of DNA at 8-, 9-, and 10-bp Sequences. M. Nelson and M. McClelland, Purification and Assay of Type II DNA Methylases. M. Nelson and I. Schildkraut, The Use of DNA Methylases to Alter the Apparent Recognition Specificities of Restriction Endonucleases. Rapid Methods for DNA Sequence Analysis. A.T. Bankier, K.M. Weston, and B.G. Barrell, Random Cloning and Sequencing by the M13/Dideoxynucleotide Chain Termination Method. G.-F. Hong, The Use of DNase I, Buffer Gradient Gel, and 35S Label for DNA Sequencing. L. Dente and R. Cortese, pEMBL: A New Family of Single-Stranded Plasmids for Sequencing DNA. T.K. Misra, DNA Sequencing: A New Strategy to Create Ordered Deletions, Modified M13 Vector, and Improved Reaction Conditions for Sequencing by Dideoxy Chain Termination Method. R. Zagursky and K. Baumeister, Construction and Use of pBR322 Plasmids That Yield Single-Stranded DNA for Sequencing. S. Henikoff, Unidirectional Digestion with Exonuclease III in DNA Sequence Analysis. S. Labeit, H. Lehrach, and R.S. Goody, DNA Sequencing Using *ga-Thiodeoxynucleotides. A. Ahmed, Use of Transposon-Promoted Deletions in DNA Sequence Analysis. R.M.K. Dale and A. Arrow, A Rapid Single-Stranded Cloning, Sequencing, Insertion, and Deletion Strategy. Z.-g. Peng and R. Wu, A New and Simple Rapid Method for Sequencing DNA. G. Volckaert, A Systematic Approach to Chemical DNA Sequencing by Subcloning in pGV451 and Derived Vectors. F.M. Pohl and S. Beck, Direct Transfer Electrophoresis Used for DNA Sequencing. L.M. Smith, R.J. Kaiser, J.Z. Sanders, and L.E. Hood, The Synthesis and Use of Fluorescent Oligonucleotides in DNA Sequence Analysis. A. Rosenthal, R. Jung, and H.-D. Hunger, Solid-Phase Methods for Sequencing of Oligodeoxynucleotides and DNA. Miscellaneous Methods. K.B. Mullis and F.A. Faloona, Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction. D. Koshland and P. Hieter, Visual Assay for Chromosome Ploidy. S. Nishimura, N. Shindo-Okada, and P.F. Crain, Isolation of Amino Acid-Specific tRNA by High-Performance Liquid Chromatography. Y. Kuchino, N. Hanyu, and S. Nishimura, Analysis of Modified Nucleosides and Nucleotide Sequence of tRNA. P.A. Krieg and D.A. Melton, In Vitro RNA Synthesis with SP6 RNA Polymerase. H. Bujard, R. Gentz, M. Lanzer, D. Stueber, M. Mueller, I. Ibrahimi, M.-T. Haeuptle, and B. Dobberstein, A T5 Promoter-Based Transcription*b1Translation System for the Analysis of Proteins in Vitro and in Vivo. G.L. Igloi and H. K"ssel, Use of Boronate-Containing Gels for Electrophoretic Analysis of Both Ends of RNA Molecules. C.L. Smith and C.R. Cantor, Purification, Specific Fragmentation, and Separation of Large DNA Molecules. G.F. Carle and M.V. Olson, Orthogonal-Field-Alternation Gel Electrophoresis. L.S. Lerman and K. Silverstein, Computational Simulation of DNA Melting and Its Application to Denaturing Gradient Gel Electrophoresis. R.M. Myers, T. Maniatis, and L.S. Lerman, Detection and Localization of Single Base Changes by Denaturing Gradient Gel Electrophoresis. J. Berman, S. Eisenberg, and B.-K. Tye, An Agarose Gel Electrophoresis Assay for the Detection of DNA-Binding Activities in Yeast Cell Extracts. T.D. Tullius, B.A. Dombroski, M.E.A. Churchill, and L. Kam, Hydroxyl Radical Footprinting: A High-Resolution Method for Mapping Protein*b1DNA Contacts. D.E. Hill, A.R. Oliphant, and K. Struhl, Mutagenesis with Degenerate Oligonucleotides: An Efficient Method for Saturating a Defined DNA Region with Base Pair Substitutions. A.R. Oliphant and K. Struhl, The Use of Random-Sequence Oligonucleotides for Determining Consensus Sequences. F.I. Lewitter and W.P. Rindone, Computer Programs for Analyzing DNA and Protein Sequences. Author Index. Subject Index.

巻冊次

pt. G ISBN 9780121821173

内容説明

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

目次

Isolation, Synthesis, and Detection of DNA and RNA:F. Guidet and P. Langridge, Megabase DNA Preparation from Plant Tissue.M. Koob and W. Szybalski, Preparing and Using Agarose Microbeads.S. Anant and K. Subramanian, Methods for Rapid Isolation of Low Molecular Weight DNA from Bacteria and Animal Cells.J.D. Harding, R.L. Bebee, and G. Gebeyehu, Rapid, Specific DNA Isolation Using Methidium-Spermine-Sepharose.R.P. Kandpal, D.C. Ward, and S.M. Weissman, Chromosome Fishing: An Affinity Capture Method for Selective Enrichment of Large Genomic DNA Fragments.L. Ulanovsky, Trapping Electrophoresis of End-Modified DNA in Polyacrylamide Gels.C.C. Lee and C.T. Caskey, Direct cDNA Cloning Using PCR.Y.L. Chiang, Direct cDNA Cloning and Screening of Mutants Using the Polymerase Chain Reaction.J. Eberwine, C. Spencer, K. Miyashiro, S. Mackler, and R. Finnell, cDNA Synthesis in Situ: Methods and Applications.Z. Cai, J.K. Pullen, R.M. Horton, and L.R. Pease, Specific Amplification of cDNA from Targeted Members of Multigene Families.H. Toyoda, Long Synthetic Oligonucleotide Probes for Gene Analysis.D.J. Kemp, Colorimetric Detection of PCR-Amplified DNA Segments Using DNA Binding Proteins.A.M. Lew and M. Panaccio, A New Technique for Preparation of DNA from Blood for PCR in a Microtiter Tray.F.F. Chehab, J. Wall, and Y.W. Kan, Amplification and Detection of Specific DNA Sequences with FluorescentPCR Primers: Application to the ~gDF508 Mutation in Cystic Fibrosis.S. Beck, Nonradioactive Detection of DNA Using Dioxetane Chemiluminescence.H.C. Birnboim, Extraction of High Molecular Weight RNA and DNA from Cultured Mammalian Cells.C. Goblet, E. Prost, K.J. Bockhold, and R.G. Whalen, Comparison of One-Tube versus Two-Step Amplification of RNA Transcripts Using Polymerase Chain Reaction.A.D. Bharucha and M.R. Ven Murthy, Characterization of Polysomes and Polysomal mRNAs by Sucrose Density Gradient Centrifugation Followed by Immobilization in Polyacrylamide Gel Matrix.G. Levesque, A.D. Bharucha, and M.R. Ven Murthy, Biochemical Manipulations of Minute Quantitiesof mRNAs and cDNAs Immobilized on Cellulose Paper Discs.J. Soh and S. Pestka, Hybrid Selection of mRNA with Biotinylated DNA.Enzymes and Methods for Cleaving and Manipulating DNA:A.S. Bhagwat, Restriction Enzymes: Properties and Use.W.A. Bickmore and A.P. Bird, Use of Restriction Endonucleases to Detect and Isolate Genes from Mammalian Cells.D. Landry, J.M. Barsomian, G.R. Feehery, and G.G. Wilson, Characterization of Type II DNA Methyltransferases.G.G. Wilson, Amino Acid Sequence Arrangements of DNA Methyltransferases.M. Nelson and M. McClelland, Use of DNA Methyltransferase/Endonuclease Enzyme Combination for Megabase Mapping of Chromosomes.A. Podhajska, S.C. Kim, and W. Szybalski, Conferring New SPecificities on Restriction Enzymes: Cleavage at any Predetermined Site by Combining an Adapter Oligodeoxynucleotide and a Class-IIS Enzyme.S.A. Strobel and P.B. Dervan, Triple Helix Mediated Single-Site Enzymatic Cleavage of Megabase Genomic DNA.M. Koob, Creating New Cleavage Specificities of Restriction Endonucleases.C.W. Fuller, Modified T7 DNA Polymerase for DNA Sequencing.Reporter Genes:J. Gould, Nondesructive Assay for GUS in Culture Media of Plant Tissue Culture.B.R. Cullen and M.H. Malim, Secreted Placental Alkaline Phosphatase: A Novel Eukaryotic Reporter Gene.D.E. Hruby and E.M. Wilson, Use of a Fluorescent Chloramphenicol Derivative as a Substrate for CAT Assays.S. de la Luna and J. Ortin, Pac Gene as an Efficient Dominant Marker and Reporter Gene in Mammalian Cells.A.R. Brasier and D. Ron, Luciferase Reporter Gene Assay in Mammalian Cells.K.R. Luehrsen, J.R. de Wet, and V. Walbot, Transient Expression Analysis in Plants Using Firefly Luciferase Reporter Gene.K. D'Halluin, M. De Block, J. Denecke, J. Janssens, J. Leemans, A. Reynaerts, and J. Botterman, Bar Gene as Selectable and Screenable Marker in Plant Engineering.A. Caplan, R. Dekeyser, and M. Van Montagu, Useful Selectable Markers for Rice Transformation.M. Witty, Thaumatin II: A Sweet Marker Gene for Use in Plants.P.J. Punt and C.A.M.J.J. van den Hondel, Transformation of Filamentous Fungi Based on Hygromycin B and Phleomycin Resistance Markers.J. Altenbuchner, P. Viell, and I. Pelletier, New Positive Selection Vectors Based on Palindromic DNA Sequences.Vectors for Cloning Genes:J. Brosius, Compilation of Superlinker Vectors.M.A. Alting-Mees, J.A. Sorge, and J.M. Short, pBluescriptR II: Multifunctional Cloning and Mapping Vectors.J.M. Short and J.A. Sorge, In Vivo Excision Properties of Bacteriophage ~gl ZAPR Expression Vectors.I.-M.M. Chiu, K. Lehtoma, and M.L. Poulin, Cloning of cDNA Inserts from Phage DNA Directly into a Plasmid Vector.A.D. Zelenetz, Efficient Construction of Complex Directional cDNA Libraries in a Sfil Cassette.G.A. Evans, K. Snider, and G.G. Hermanson, Use of Cosmids and Arrayed Clone Libraries for Genome Analysis.J.C. Pierce and N.L. Sternberg, Using the Bacteriophase P1 System to Clone High Molecular Weight (HMW) Genomic DNA.K.D. Tartof, Cloning Vectors and Techniques for Exo-Hyb Restriction Mapping.R.H. Reeves, W.J. Pavan, and P. Hieter, YAC Modification and Manipulation.D.R. Smith, A.P. Smyth, and D.T. Moir, Copy Number Amplification of Yeast Artificial Chromosomes.R. Allshire, Manipulation of Large Minichromosomes in S. pombe with Liposome Enhanced Transformation.A.D. Miller, D.G. Miller, J.V. Garcia, and C.M. Lynch, Use of Retroviral Vectors for Gene Transfer and Expression.Author Index.Subject Index.

巻冊次

pt. H ISBN 9780121821180

内容説明

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

目次

Vectors for Expressing Cloned Genes:G. Sczakiel and K. Maeda, Vector pPLEX for Expression of Nonfusion Polypeptides in Escherichia coli.H. Orum and L.K. Poulsen, In-Frame Gene Fusion.S. Udaka and H. Yamagata, High Level Secretion of Heterologous Proteins by Bacillus brevis.S.E. Kane and M.M. Gottesman, Use of Multidrug Resistance Gene in Mammalian Expression Vectors.A. Lieber, V. Sandig, W. Sommer, S. B~adahring, and M. Strauss, Stable High Level Gene Expression in Mammalian Cells by T7 Phage RNA Polymerase.R. T~adopfer, C. Maas, C. H~adoricke-Grandpierre, J. Schell, and H.-H. Steinbiss, Expression Vectors for High Level Gene Expression in Dicotyledonous and Monocotyledonous Plants.K.D. MacFerrin, L. Chen, M.P. Terranova, S.L. Schreiber, and G.L. Verdine, Overproduction of Proteins Using the Expression-Cassette Polymerase Chain Reaction.T.L. Pauls and M.W. Berchtold, Efficient cDNA Amplification and Expression Using PCR Technology.V.I. Baranov and A.S. Spirin, Gene Expression in a Cell-Free System on the Preparative Scale.B. Dasmahapatra, Cell-Free Expression Vector: Use of Insect Virus Translational Initiation Signal for in Vitro Gene Expression.C. Coleclough, Cell-Free Expression of Large Collections of cDNA Clones Using Positive Selection, ~gl Phage Vectors.Mutagenesis and Gene Disruption:B. Hofer and B. K~aduhlein, Site-Specific Mutagenesis in Plasmids: A Gapped Circle Method.D.B. Olsen, J.R. Sayers, and F. Eckstein, Site-Directed Mutagenesis of Single-Stranded and Double-Stranded DNA by the Phosphorothioate Approach.L.-J. Zhao, Q.X. Zhang, and R. Padmanabhan, PCR-Based Point Mutagenesis Protocol.G.P. Smith and J.K. Scott, Libraries of Peptides and Proteins Displayed on Filamentous Phage.M.M.Y. Waye, Use of M13 Ping-Pong Vectors and T4DNA Polymerase in Oligodeoxynucleotide-Directed Mutagenesis.R.M. Horton, S.N. Ho, J.K. Pullen, H.D. Hunt, Z. Cai, and L.R. Pease, Gene Splicing by Overlap Extension.C. Batt, Y. Cho, and A.C. Jamieson, Selection of Oligodeoxynucleotide-Directed Mutants.J.A. Saleeba and R.G.H. Cotton, Chemical Cleavage of Mismatch to Detect Mutations.D.C. Kaslow and D.J. Rawlings, Restriction Sites into Double-Stranded Plasmid DNA.T.C. Huffaker, Expression and Screening in Yeast of Genes Mutagenized in Vitro.W.S. Reznikoff, R. Jilk, M.P. Krebs, J.C. Makris, P.L. Nordmann, M. Weinreich, and T. Wiegand, Tn5 lacZ Translation Fusion Element: Isolation and Analysis of Tranposition Mutants.Screening Libraries, Identifying and Mapping Genes:J. Chao, K.X. Chai, and L. Chao, Screening of cDNA Library Using Radiolabeled Antigen.P. Karlovsky, Alternatives to X-Galactopyranoside in Screening Recombinant Clones Based on pUC-Derived Plasmid Vectors.G.V. Paddock, Rapid Colony Hybridization on Paper Using Oligonucleotide Probes.A. Poustka, Large Insert Linking-Clone Libraries: Construction and Use.A. Poustka, Construction and Use of Chromosome Jumping Libraries.C.Y. Yu, L.-c. Wu, L. Buluwela, and C. Milstein, Cosmid Cloning and Walking to Map Human CD1 Leukocyte Differentiation AntigenGenes:I. Bronstein, J.C. Voyta, O.J. Murphy, R. Tizard, C.W. Ehrenfels, and R.L. Cate, Detection of DNA in Southern Blots with Chemiluminescence.H.S. Rye, S. Yue, M.A. Quesada, R.P. Haugland, R.A. Mathies, and A.N. Glazer, Picogram Detection of Stable Dye-DNA Intercalation Complexes with a Two-Color Laser-Excited Confocal Fluoresence Gel Scanner.C. Cantrell and E. Lai, Alginate as Matrix Support for Yeast Artificial Chromosome Cloning.V. Shyamala and G.F.-L. Ames, Genome Walking by Single Specific Primer-Polymerase Chain Reaction.M.C.-T. Hu and N. Davidson, Mapping Transcription Start Points with T4 DNA Polymerase.Methods for Transforming Animal and Plant Cells:H. Potter, Application of Electroporation in Recombinant DNA Technology.J.R. Simon, Transformation of Intact Yeast Cells by Electroporation.J.C. Sanford, F.D. Smith, and J.A. Russell, Optimizing Biolistic Process for Biological Applications.J.E. Boynton and N. Gillham, Chloroplast Transformation in Chlamydomonas.H. Daniell, Foreign Gene Expression in Chloroplasts on Higher Plants Mediated by Tungsten Particle Bombardment.G.P. Mazzara, Generation and Analysis of Vaccinia Virus Recombinants.A.D. Miller, D.G. Miller, J.V. Garcia, and C.M. Lynch, Use of Retroviral Vectors for Gene Transfer amd Expression.J.-P. Loeffler and J.-P. Behr, Gene Transfer into Primary and Established Mammalian Cell Lines with Lipopolyamine-Coated DNA.M. Cotten, E. Wagner, and M.L. Birnstiel, Receptor-Mediated Transport of DNA into Eukaryotic Cells.V.J. Dwarki, R.W. Malone, and I.M. Verma, Cationic Liposome-Mediated RNA Transfection.Author Index.Subject Index.

巻冊次

pt. I ISBN 9780121821197

内容説明

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

目次

Methods for Sequencing DNA:U.B. Gyllensten and M. Allen, Methods for Sequencing of in Vitro Amplified DNA.L.F. Landweber and M. Kreitman, Strategy for Producing Single-Stranded DNA in Polymerase Chain Reaction for Direct Genomic Sequencing.J.G. Lawrence, D.L. Hartl, and H. Ochman, Sequencing Products of Polymerase Chain Reaction.R.L. Dorit, One-Sided PCR for Amplification and Sequencing cDNA.Z. Liu and P.B. Hackett, Reverse Cloning Procedure for Rapid Generation of Subclones for DNA Sequencing.W. Shen and M.M.Y. Waye, Simple Method for Generating Unidirectional Deletion Mutants for DNA Sequencing and Protein Engineering.K. Kretz and J.S. O'Brien, Direct Sequencing of PCR Products from Low-Melting Temperature Agarose.D.B. Olsen, G. Wunderlich, A. Uy, and F. Eckstein, Direct Sequencing of Polymerase Chain Reaction Products.J.P. Schofield, D.S.C. Jones, and M. Vaudin, Fluorescent and Radioactive Solid-Phase Dideoxy Sequencing of PCR Products in Microtiter Plates.Z. Du, L. Hood, and R.K. Wilson, Automated Fluorescent DNA Sequencing of PCR Products.R. Kaiser, T. Hunkapiller, C. Heiner, and L. Hood, Specific-Primer Directed DNA Sequence Analysis Using Automated Four-Color Fluorescence Detection and Labeled Primers.J.A. Luckey, H. Drossman, T. Kostichka, and L.M. Smith, High Speed DNA Sequencing by Capillary Gel Electrophoresis.V. Smith, M. Craxton, A.T. Bankier, C.M. Brown, W.D. Rawlinson, M.S. Chee, and B.G. Barrell, Microtiter Methods for Preparation and Fluorescent Sequencing of M13 Clones.P. Richterich and G.M. Church, DNA Sequencing with Direct Transfer Electrophoresis and Nonradioactive Detection.B.L. Iverson and P.B. Dervan, Adenine Specific Chemical DNA Sequencing Reaction.J. Kraus and T. Tahara, Direct DNA Sequencing of PCR Amplified Genomic DNA by Maxam-Gilbert Method.D.L. Steffens and R.W. Gross, Direct Sequencing of ~gggt11 Clones.T.L. McGuigan, K.J. Livak, and S. Brenner, DNA Fingerprinting by Sampled Sequencing.B.R. Krishnan, D. Kersulyte, I. Brikun, H.V. Huang, C.M. Berg, and D.E. Berg, Transposon- and PCR-Based Sequencing of DNAs cloned in ~gl Phage.C.M. Berg, G. Wang, L.D. Strausbaugh, and D.E. Berg, Transposon-Facilitated Sequencing of DNAs Cloned in Plasmids.PCR for Amplifying and Manipulating DNA:H. Ochman, F.J. Ayala, and D.L. Hartl, Use of Polymerase Chain Reaction to Amplify Segments outside Boundaries of Known Sequences.M.J. Geiger, M. Bull, D.D. Eckels, and J. Gorski, Modified Method for "One-Way PCR": Amplification of cDNA from mRNA with Unknown 5' Ends.D.M. Dorfman, Amplification of Bacteriophage Library Inserts Using Polymerase Chain Reaction (PCR).M. Frohman, Thermal RACE (Rapid Amplification of cDNA Ends).V. Jung and S. Pestka, Efficient Cloning of PCR-Generated DNA Containing Terminal Restriction Endonuclease Recognition Sites.G.S. Sandhu, J.W. Precup, and B.C. Kline, Rapid One-Step Characterization of Recombinant DNA Vectors by PCR Analysis of Whole Cells.E. Kawasaki, R. Saiki, and H. Erlich, Genetic Analysis Using PCR-Amplified DNA and Immobilized Oligonucleotide Probes: Reverse Dot Blot Typing.G. Sarkar and S.S. Sommer, Removal of DNA Contamination in PCR Reagents by UV Irradiation.C.D.K. Bottema, G. Sarkar, J.D. Cassady, S. Li, C.M. Dutton, and S.S. Sommer, PCR Amplification of Specific Alleles: General Method of Rapidly Detecting Mutations, Polymorphisms, and Haplotypes.C.A. Dionne and M. Jaye, Chromosome Assignment by PCR Techniques.M.D. Jones, Reverse Transcription of mRNA by Thermus aquaticus DNA Polymerase Followed by PCR Amplification.M. Becker-Andr~aae, Determination of Absolute Levels of mRNA by PCR Aided Transcript Titration Assay (PATTY).R.H. Scheuermann and S.R. Bauer, PCR-Based mRNA Quantification Using an Internal Standard: Method for Analysis of Oncogene Expression.A.-C. Syv~adanen and H. S~adoderlund, Quantitation of Polymerase Chain Reaction Products by Affinity-Based Collection.Methods for Detecting DNA-Protein Interaction:M.G. Peterson and R. Tjian, Cross-Species PCR: Cloning of TATA Box Binding Proteins.O.S. Gabrielsen and J. Huet, Magnetic DNA Affinity Purification of a Yeast Transcription Factor.A.M. Lew, V.M. Marshall, and D.J. Kemp, Affinity Selection of PCR Products by DNA Binding Proteins.D.B. Tully and J.A. Cidlowski, Application of Protein Blotting Procedures to Evaluate Interactions of Steroid Receptors with DNA.H. Singh, Using Specific Recognition Site Probes to Identify Genes Coding for DNA Binding Proteins.P.B. Becker, F. Weih, and G. Sch~adutz, Footprinting of DNA-Binding Proteins in Intact Cells.M.J. Longley and D.W. Mosbaugh, In Situ Detection of DNA Metabolizing Enzymes Following Polyacrylamide Gel Electrophoresis.J.-C. Lelong, Simultaneous Characterization of DNA Binding Proteins and their Specific Genomic DNA Target Sites.Other Methods:C.T. Chung and R.H. Miller, One-Step Method for Preparation and Storage of Competent Escherichia coli Cells.P.A. Whittaker, Storage of Unamplified Phage Libraries on Nylon Filters.R. Padmanabhan, R. Padmanabhan, T. Howard, M.M. Gottesman, and B.H. Howard, Use of Magnetic Affinity Cell Sorting to Isolate Transiently Transfected Cells, Multidrug Resistant Cells, Somatic Cell Hybrids, and Virally Infected Cells.M. Yi and N. Ichikawa, and P.O.P. Ts'o, General Procedures for Detection of Genomic DNA Rearrangements at Restriction Fragment Level.Y.-R. Song, Z.-H. Ye, and J.E. Varner, Tissue Print Hybridization on Membrane for Localization of mRNA in Plant Tissue.G.I. Cassab, Localization of Cell Wall Proteins Using Tissue-Print Western Blot Technique.T.J. Guilfoyle, B.A. McClure, M.A. Gee, and G. Hagen, Tissue Print Hybridization: Simple Technique for Detecting RNA Directly.M.W. Mather, J.A. Keightley, and J. Fee, Recovery and Cloning of Genomic DNA Fragments from Dried Agarose Gels.J.G.K. Williams, M.K. Hanafey, J.A. Rafalski, and S.V. Tingey, Genetic Analysis Using RAPD Markers.Author Index.Subject Index.

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詳細情報

• NII書誌ID(NCID): BA01188399
• ISBN:
  • 012181968X
  • 0121820009
  • 0121820017
  • 0121820548
  • 0121820556
  • 0121820564
  • 012182117X
  • 0121821188
  • 0121821196
• LCCN: 79026584
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York ; Tokyo
• ページ数/冊数: v.
• 大きさ: 24 cm
• 分類:
  • NLM : QU58
  • NDLC : RA84
  • NDC8 : 464.5
  • NDC8 : 464
  • LCC : QP601
  • LCC : QH442
  • DC19 : 574.1/925 s
  • DC19 : 574.87/3282
• 件名:
  • LCSH : Recombinant DNA
• 親書誌ID:
  • BA00197543