書誌事項

Culturing nerve cells

edited by Gary Banker and Kimberly Goslin

(Cellular and molecular neuroscience series)

MIT Press, c1991

大学図書館所蔵 件 / 25

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注記

Includes bibliographical references and index

"a bradford book"

内容説明・目次

内容説明

This do-it-yourself manual describes complete recipes and protocols for biological scientists, particularly neurobiologists, who want to learn how to grow neurons in culture and how to do it well. It also provides an understanding of the principles behind the protocols and takes into account the details that can make the difference between success and failure by asking experienced culturists to share their technical expertise. Introductory chapters present a brief course on culturing all types of nerve cells - including the kinds of cultures available and which might be best for a specific application - methods for cell dissociation, culture media and substrates, and a description of the techniques needed to characterize and study cultures. More than 20 experts then describe the culture systems that they have developed, including the details of each protocol as well as details of the many problems which may be encountered.

目次

  • Part 1 A user's guide: getting started. Part 2 General principles: types of nerve cell cultures, their advantages and limitations
  • primary dissociated cell cultures of neuronal tissue
  • characterizing and studying neuronal cultures. Part 2 Culture of specific cell-types: isolated chick neurons for the study of axonal growth
  • culturing spinalneurons and muscle cells from xenopus embryos
  • culturing the large neurons of aplysia
  • tissue culture of mammalian autonomic neurons
  • methodologies for the culture and experimental use of the PC12 rat pheochromocytoma cell line
  • cell culture of neocortex and basal forebrain from postnatal rats
  • rat hippocampal neurons in low-density culture
  • cerebellar cells in culture
  • astroglia in culture
  • tissue culture methods for the study of myelination
  • organotypic slice cultures of neural tissue.

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