Analyzing DNA
著者
書誌事項
Analyzing DNA
(Genome analysis : a laboratory manual / editors, Eric D. Green ... [et al.], v. 1)
Cold Spring Harbor Laboratory Press, c1997
- : comb.
- : cloth.
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Assuming only a basic knowledge of molecular biology, this is the first in a series of manuals which explains how to clone, manipulate, analyze and sequence large segments of DNA, and relate expressed sequence to phenotypic variation. The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure, and solutions.
目次
- Purifying and analyzing genomic DNA
- preparation, manipulation and mapping of HMW DNA
- PCR in genome analysis
- fluorescence-based DNA sequencing
- shotgun sequencing
- transposon-mediated DNA sequencing
- computational analysis of DNA and protein sequences. Appendices: common reagents
- basic procedures
- safety precautions
- useful facts
- suppliers.
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