At the bench : a laboratory navigator

著者

書誌事項

At the bench : a laboratory navigator

by Kathy Barker

Cold Spring Harbor Laboratory Press, c1998

大学図書館所蔵 件 / 全22件

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

A handbook for living and working in the laboratory. It discusses: how research groups work on a human level - and how to fit in; what equipment is essential, and how to use it properly; how to get started and get organized; how to set up an experiment; how to handle and use data and reference sources; and how to present yourself and your results - in print and in person. It offers advice, moral support, social etiquette, and professional assurance, along with step-by-step instructions for those basic laboratory procedures that experienced investigators know - but novices do not.

目次

  • Part 1: general lab organization and procedures - the big picture, laboratory personnel, lab routines, what to expect the first week, what not to do the first week, survival through common sense and courtesy, nonnegotiable safety rules, references and resources
  • laboratory set-up and equipment - layout of the land, using the equipment, how to buy new equipment, references and resources
  • getting started and staying organized - setting up a functional lab bench, setting up the command centre, references and resources. Part 2: how to set up an experiment - philosophical considerations, planning and experiment, interpreting results, references and resources
  • laboratory notebooks - type and format, content, maintenance, ethics, references and resources
  • presenting yourself and your data - communication tips, oral presentations, written presentations, references and resources. Part 3: making reagents and buffers - determining what you need, calculating what you need, weighing and mixing, measuring pH, sterilizing solutions, storing buffers and solutions, references and resources
  • storage and disposal - emergency storage, storing reagents, aliquoting, refrigerator and freezers, discarding lab waste, references and resources
  • working without contamination - when to use sterile technique, sterile techniques, protecting the investigator
  • sterile technique in the Class II biosafety cabinet
  • references and resources
  • eukaryotic cell culture - types of cultures and cell lines, observing cells, obtaining cells, culturing frozen cells, cell maintenance, freezing and storage of cells, contamination, CO2 incubators and tanks, references and resources
  • bacteria - setting up, working rules, obtaining bacteria, growth and maintenance, storage, contamination, reference and resources
  • DNA, RNA and protein - tips, DNA, RNA, protein, references and resources
  • radioactivity - the properties of radioactive elements, how to obtain radioisotopes, doing radioactive experiments, experimental detection of radiation, storage, disposal, alternatives to radioactivity, references and resources
  • centrifugation - background, working rules, how to spin, gradients, centrifuge and rotor maintenance, resources and references
  • electrophoresis, microscope.

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