Molecular cloning : a laboratory manual

著者

書誌事項

Molecular cloning : a laboratory manual

Joseph Sambrook, David W. Russell

Cold Spring Harbor Laboratory Press, c2001

3rd ed

  • v. 1 : pbk
  • v. 2 : pbk
  • v. 3 : pbk
  • v. 1 : cloth
  • v. 2 : cloth
  • v. 3 : cloth

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

目次

Chapter 1. Plasmids and Their Usefulness in Molecular Cloning Chapter 2. Bacteriophage lambda and Its Vectors Chapter 3. Working with Bacteriophage M13 Vectors Chapter 4. Working with High-capacity Vectors Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction Chapter 9. Preparation of Radiolabeled DNA and RNA Probes Chapter 10. Working with Synthetic Oligonucleotide Probes Chapter 11. Preparation of cDNA Libraries and Gene Identification Chapter 12. DNA Sequencing Chapter 13. Mutagenesis Chapter 14. Screening Expression Libraries Chapter 15. Expression of Cloned Genes in Escherichia coli Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells Chapter 18. Protein Interaction Technologies Appendices

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